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Multiplex I cfDNA Reference Standard Set in Synthetic Matrix

Developed using clinically-relevant mutations in an artificial matrix to give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in synthetic matrix contains 8 onco-relevant mutations to measure your extraction efficiency. Put the control of your workflow back in your hands. 

Note: This reference standard set, in a new synthetic matrix, is now compatible with more cell free DNA extraction methods, see HD917.

The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.

For information on specific gene copies detected in each product batch, download the product insert from the resources tab.

Technical data

Format: cfDNA

Genes covered: EGFR, KRAS, NRAS, PIK3CA

Cosmics: 1,500+

Allelic frequencies: 5%, 1%, 0.1% and 0% (100% wild type)

Buffer: Synthetic matrix

Expected DNA yield: ≥120 ng DNA using the QIAamp® Circulating Nucleic Acid Kit

Product information

Fragment size: 170bp

Unit size: 400ng per 1ml (4 vials total)

Verified mutations

100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD815)

Chromosome

Gene

Variant

Expected Allelic Frequency

7p12

EGFR

L858R

0.00%

7p12

EGFR

ΔE746 - A750

0.00%

7p12

EGFR

T790M

0.00%

7p12

EGFR

V769 - D770insASV

0.00%

12p12.1

KRAS

G12D

0.00%

1p13.2

NRAS

Q61K

0.00%

1p13.2

NRAS

A59T

0.00%

3q26.3

PIK3CA

E545K

0.00%

5% Multiplex I cfDNA Reference Standard (Part No.: HD812)

Chromosome

Gene

Variant

Expected Allelic Frequency

7p12

EGFR

L858R

5.0%

7p12

EGFR

ΔE746 - A750

5.0%

7p12

EGFR

T790M

5.0%

7p12

EGFR

V769 - D770insASV

5.0%

12p12.1

KRAS

G12D

6.3%

1p13.2

NRAS

Q61K

6.3%

1p13.2

NRAS

A59T

6.3%

3q26.3

PIK3CA

E545K

6.3%

1% Multiplex I cfDNA Reference Standard (Part No.: HD813)

Chromosome

Gene

Variant

Expected Allelic Frequency

7p12

EGFR

L858R

1.0%

7p12

EGFR

ΔE746 - A750

1.0%

7p12

EGFR

T790M

1.0%

7p12

EGFR

V769 - D770insASV

1.0%

12p12.1

KRAS

G12D

1.3%

1p13.2

NRAS

Q61K

1.3%

1p13.2

NRAS

A59T

1.3%

3q26.3

PIK3CA

E545K

1.3%

0.1% Multiplex I cfDNA Reference Standard (Part No.: HD814)

Chromosome

Gene

Variant

Expected Allelic Frequency

7p12

EGFR

L858R

0.10%

7p12

EGFR

ΔE746 - A750

0.10%

7p12

EGFR

T790M

0.10%

7p12

EGFR

V769 - D770insASV

0.10%

12p12.1

KRAS

G12D

0.13%

1p13.2

NRAS

Q61K

0.13%

1p13.2

NRAS

A59T

0.13%

3q26.3

PIK3CA

E545K

0.13%

Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency)
Download additional information on variants present in the parental cell lines: Exome Sequencing Data

General information

Storage: 4°C

Expiration: See all product shelf life information

Quality control

Fragmentation size: D1000 DNA ScreenTape assay

Allelic frequency: Droplet Digital™ PCR

Quantification: Qubit dsDNA BR Assay (post-fragmentation)