Multiplex I cfDNA Reference Standard Set in Synthetic Matrix
Developed using clinically-relevant mutations in an artificial matrix to give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in synthetic matrix contains 8 onco-relevant mutations to measure your extraction efficiency. Put the control of your workflow back in your hands.
The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.
For information on specific gene copies detected in each product batch, download the product insert from the resources tab.
Technical data
Format: cfDNA
Genes covered: EGFR, KRAS, NRAS, PIK3CA
Cosmics: 1,500+
Allelic frequencies: 5%, 1%, 0.1% and 0% (100% wild type)
Buffer: Synthetic matrix
Expected DNA yield: ≥120 ng DNA using the QIAamp® Circulating Nucleic Acid Kit
Product information
Fragment size: 170bp
Unit size: 400ng per 1ml (4 vials total)
Verified mutations
|
100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD815) |
|||
|
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
|
7p12 |
EGFR |
L858R |
0.00% |
|
7p12 |
EGFR |
ΔE746 - A750 |
0.00% |
|
7p12 |
EGFR |
T790M |
0.00% |
|
7p12 |
EGFR |
V769 - D770insASV |
0.00% |
|
12p12.1 |
KRAS |
G12D |
0.00% |
|
1p13.2 |
NRAS |
Q61K |
0.00% |
|
1p13.2 |
NRAS |
A59T |
0.00% |
|
3q26.3 |
PIK3CA |
E545K |
0.00% |
|
5% Multiplex I cfDNA Reference Standard (Part No.: HD812) |
|||
|
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
|
7p12 |
EGFR |
L858R |
5.0% |
|
7p12 |
EGFR |
ΔE746 - A750 |
5.0% |
|
7p12 |
EGFR |
T790M |
5.0% |
|
7p12 |
EGFR |
V769 - D770insASV |
5.0% |
|
12p12.1 |
KRAS |
G12D |
6.3% |
|
1p13.2 |
NRAS |
Q61K |
6.3% |
|
1p13.2 |
NRAS |
A59T |
6.3% |
|
3q26.3 |
PIK3CA |
E545K |
6.3% |
|
1% Multiplex I cfDNA Reference Standard (Part No.: HD813) |
|||
|
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
|
7p12 |
EGFR |
L858R |
1.0% |
|
7p12 |
EGFR |
ΔE746 - A750 |
1.0% |
|
7p12 |
EGFR |
T790M |
1.0% |
|
7p12 |
EGFR |
V769 - D770insASV |
1.0% |
|
12p12.1 |
KRAS |
G12D |
1.3% |
|
1p13.2 |
NRAS |
Q61K |
1.3% |
|
1p13.2 |
NRAS |
A59T |
1.3% |
|
3q26.3 |
PIK3CA |
E545K |
1.3% |
|
0.1% Multiplex I cfDNA Reference Standard (Part No.: HD814) |
|||
|
Chromosome |
Gene |
Variant |
Expected Allelic Frequency |
|
7p12 |
EGFR |
L858R |
0.10% |
|
7p12 |
EGFR |
ΔE746 - A750 |
0.10% |
|
7p12 |
EGFR |
T790M |
0.10% |
|
7p12 |
EGFR |
V769 - D770insASV |
0.10% |
|
12p12.1 |
KRAS |
G12D |
0.13% |
|
1p13.2 |
NRAS |
Q61K |
0.13% |
|
1p13.2 |
NRAS |
A59T |
0.13% |
|
3q26.3 |
PIK3CA |
E545K |
0.13% |
Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency)
Download additional information on variants present in the parental cell lines: Exome Sequencing Data
General information
Storage: 4°C
Expiration: See all product shelf life information
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit dsDNA BR Assay (post-fragmentation)
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