The Human Accell Ion Channel library contains siRNA reagents targeting transmembrane proteins having known or predicted ion channel activity. These include both ligand-gated channels, such as the nicotinic acetylcholine receptors, and voltage-gated channels, such as those that regulate the passage of potassium, sodium, and calcium during polarization and depolarization of the cell membrane.

Accell siRNA achieves what no other RNAi product can claim: delivery into difficult-to-transfect cells without transfection reagents, virus, or electroporation. Researchers around the world are achieving targeted gene silencing in cells that had previously been beyond the reach of conventional RNAi products due to toxicity caused by transfection reagents or undesirable viral responses.

Highlights

• Accell siRNA enters cells without the need for transfection reagents, virus (or viral vectors), or instruments
• Proven performance in neuronal, immunological, primary, and other difficult-to-transfect cell types
• Extended-duration knockdown with optimized continuous application
• Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96 well plates

Gene Targets

For a complete list of target genes in this siRNA Library, please contact Scientific Support.

Experimental Considerations

• Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration
• Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended
• Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown