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shMIMIC Lentiviral microRNA Pooled Libraries
Perform functional screening of hundreds or thousands of microRNAs without high-throughput automation
Optimal microRNA expression for high-confidence functional analysis screening
Target every human and mouse microRNA detailed in the miRBase database. Stable expression of a mature microRNA, using shMIMIC Lentiviral microRNA pooled libraries.
Screen large numbers of microRNAs without automation. shMIMIC pooled libraries available for all human and mouse miRBase matures, as well as a human/mouse conserved collection.
Library construction, pooling techniques, and high-throughput sequencing-compatible screening workflows validated to ensure reproducibility and accurate hit identification. Learn about optimal library design and the pooled screening workflow on our Pooled Lentiviral Screening Libraries page.
Highlights:
- Innovative vector design for robust microRNAs expression from shMIMIC lentiviral microRNAs
- SMARTchoice options - select the promoter and reporter (or no reporter!) for optimal performance in your experiment
- The constructs are designed to preserve the function of the miRNA repression but may deviate slightly from the mirBase sequence
You may also select shMIMIC Inducible microRNA pooled libraries for tight regulation of expression from the Tet-On® 3G transactivator protein
Each shMIMIC lentiviral library includes:
- 30 Non-targeting controls
- 80 species-specific positive control shRNAs targeting 10 common viability and reference genes (8 shRNAs each)
- Data file including construct sequences, miRBase gene IDs, and counts per millions of mapped reads
| shMIMIC microRNA Pooled Library | Number of mature microRNAs | Number of unique designs | Number of pools × number of constructs per pool1 |
|---|---|---|---|
| Human | 2580 | 2555 | 1 pool × 2665 constructs |
| Mouse | 1913 | 1896 | 1 pool × 2006 constructs |
| Human-Mouse Conserved | 355 human + 341 mouse | 386 | 1 pool x 496 constructs |
Reagents recommended in our validated protocol:
- Vector-matched SMARTvector Non-targeting control for transduction optimization
- SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for:
- Efficient PCR amplification of genomic DNA with minimal bias
- High-throughput multiplexed Next Gen Sequencing for hit identification
Before ordering, download these valuable tools to plan your pooled lentiviral screen and calculate the amounts of components required:
- SMARTvector & shMIMIC Lentiviral Pooled Libraries Technical Manual
- Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet
Important Notice
The shMIMIC Lentiviral microRNA Pooled Libraries are solely for internal research use (as set forth in the Product Terms and Conditions) in laboratories where the containment measures stated below and in applicable laws and regulations are met. Reagents may not be used for diagnostic, therapeutic or other commercial purposes and may not to be administered to humans for any purpose or to animals for therapeutic purposes. SMARTvector Lentiviral shRNA Pooled Libraries are provided as lentiviral particles are replication-incompetent, self-inactivating (SIN) and non-pathogenic (do not cause infectious human disease).
Any investigator who purchases lentiviral particle products is responsible for consulting with their institution's health and biosafety personnel for specific guidelines on the handling of lentiviral vector particles. Furthermore, each investigator is fully responsible for obtaining the required permissions for research using and the acceptance of replication-incompetent SIN lentiviral vectors and replication-defective lentiviral particles into their local jurisdiction and institution.
