Human and mouse genome-wide collections available as glycerol stock.
TRC whole genome libraries for human or mouse, assembled using innovative bioinformatic tools to reflect the most up-to-date genetic and ontological information. Offered as glycerol stocks of shRNA clones arrayed in 96-well plates.
Benefits
- Cloned into lentiviral vectors
- Amenable to in vitro and in vivo applications
- Lentiviral vector enables transduction of primary and non-dividing cells
- Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html
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TRC lentiviral shRNA libraries
Simple hairpin shRNAs in the pLKO.1 lentiviral vector designed by The RNAi Consortium (TRC)
Bacterial glycerol stock
Bacterial culture transformed with a plasmid vector, grown in the presence of glycerol for freezer storage and supplied in single tubes (at least three constructs per target gene) and predefined or custom libraries.
Arrayed library format
Genome-scale, predefined or custom libraries of bacterial glycerol stocks arrayed in 96-well microtiter plates.
Gene families and pathways
Predefined libraries arranged by gene family and/or function and arrayed in 96-well microtiter plates.
Which shRNA reagent is best for you?
Use the table below to assist you in determining the right shRNA reagents for your experimental needs.
| SMARTvector shRNA | GIPZ | TRIPZ | TRC | |
|---|---|---|---|---|
| Species | Human, Mouse, Rat | Human, Mouse | Human | Human, Mouse |
| Promoter | Choice of 7 constitutive* and 4 inducible promoters | Human CMV Pol II | TRE-min-CMV | U6 Pol III |
| Vector backbone | Lentiviral | Lentiviral | Lentiviral | Lentiviral |
| Stem-loop format | MicroRNA-adapted | MicroRNA-adapted | MicroRNA-adapted | Simple |
| Fluorescent reporter gene | GFP/RFP | GFP | RFP | |
| Guaranteed silencing** | ||||
| In vivo RNAi | ||||
| Create stable cell lines | ||||
| Recommended for primary and non-dividing cells | ||||
| Inducible expression | ||||
| Whole genome library availability | Human, Mouse, Rat | Human, Mouse | Human | Human |
| Formats | Bacterial glycerol stock High-titer lentiviral particles Gene families and pathways | Bacterial glycerol stock Arrayed library format Gene families and pathways High-titer lentiviral particles shRNA Starter Kit | Bacterial glycerol stock Arrayed library format Gene families and pathways shRNA Starter Kit | Bacterial glycerol stock Arrayed library format Gene families and pathways |
*For SMARTvector, GIPZ, and TRIPZ lentiviral shRNAs, at least one out of three constructs is guaranteed to reduce target mRNA levels by 70% or more when used in combination with the appropriately matched non-targeting and positive controls.