Human ON-TARGETplus siRNA Library - Transcription Factors
siRNA designed and modified for greater specificity
The Human ON-TARGETplus siRNA Transcription factors Library targets proteins that bind DNA in a sequence-specific manner and thereby control the transfer, or transcription, of genetic information from DNA to RNA. Transcription factors perform this function alone, or with other proteins in a complex, by promoting (as an activator) or blocking (as a repressor) the recruitment of RNA polymerase to specific genes.
siRNA designs and modifications reduce off-target effects while maintaining high silencing potency for high-confidence screening results.
Highlights
- Patented dual-strand ON-TARGETplus modification pattern on all siRNAs to reduce off-targets
- Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
- Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
- Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96-well or 384-well plates
- Guaranteed target gene knockdown (see Specifications tab)
Our siRNA knockdown guarantee
ON-TARGETplus and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 nM siRNA).
Note: Most ON-TARGETplus and ON-TARGETplus siRNA products are highly functional at 5 to 25 nM working concentration.
Gene Targets
For a complete list of target genes in this siRNA Library, please contact Technical Support or your local Sales Representative.
