- Transfection and ancillary reagents
- siGLO Lamin A/C Control siRNA (Rat)
siGLO Lamin A/C Control siRNA (Rat)
Correlate fluorescence uptake and silencing activity with a single control
siGLO Lamin A/C Control siRNA is a validated positive control, guaranteed to silence Lamin A/C in rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.
Lamins are intermediate filament-type proteins, which form major components of the nuclear lamina. Lamins A and C have close sequence homology and are derived by differential splicing and alternative polyadenylation from one gene. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.
Highlights
- Targets accession number: NM_001002016 (Rat)
- Labeled wtih Cy3
- Absorbance/Emission Max is 547/563 nm
- A Cy3, Rhodamine or PE filter can be used
- Significantly extended intensity of the fluorescence signal (7 days or longer)
- Positive correlation of cytoplasmic fluorescence with transfection efficiency
To request a larger quantity or in vivo processing of this control product, please contact Technical Support.
Cytoplasmic fluorescence confirms transfection with siGLO Lamin A/C control siRNA
siGLO Lamin A/C control (human; 50nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 µg/100µL well). After 48 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).
Shipping Condition | Ambient | |
Storage Conditions | -20 C | |
Stability at Recommended Storage Conditions | At least 12 months | |
Hazardous | No |
Product inserts
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