siGLO Lamin A/C Control siRNA is a validated positive control, guaranteed to silence Lamin A/C in rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.
Lamins are intermediate filament-type proteins, which form major components of the nuclear lamina. Lamins A and C have close sequence homology and are derived by differential splicing and alternative polyadenylation from one gene. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.
- Targets accession number: NM_001002016 (Rat)
- Labeled wtih Cy3
- Absorbance/Emission Max is 547/563 nm
- A Cy3, Rhodamine or PE filter can be used
- Significantly extended intensity of the fluorescence signal (7 days or longer)
- Positive correlation of cytoplasmic fluorescence with transfection efficiency
To request a larger quantity or in vivo processing of this control product, please contact Technical Support.
Cytoplasmic fluorescence confirms transfection with siGLO Lamin A/C control siRNA
siGLO Lamin A/C control (human; 50nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 µg/100µL well). After 48 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).
|Storage Conditions||-20 C|
|Stability at Recommended Storage Conditions||At least 12 months|
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.