siGLO Lamin A/C Control siRNA (Rat)
Correlate fluorescence uptake and silencing activity with a single control
siGLO Lamin A/C Control siRNA is a validated positive control, guaranteed to silence Lamin A/C in rat cells. This control siRNA is chemically modified to significantly extend siRNA stability and fluorescence compared to conventional siRNAs similarly labeled with fluorophores.
Lamins are intermediate filament-type proteins, which form major components of the nuclear lamina. Lamins A and C have close sequence homology and are derived by differential splicing and alternative polyadenylation from one gene. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability.
- Targets accession number: NM_001002016 (Rat)
- Labeled wtih Cy3
- Absorbance/Emission Max is 547/563 nm
- A Cy3, Rhodamine or PE filter can be used
- Significantly extended intensity of the fluorescence signal (7 days or longer)
- Positive correlation of cytoplasmic fluorescence with transfection efficiency
To request a larger quantity or in vivo processing of this control product, please contact Technical Support.
Cytoplasmic fluorescence confirms transfection with siGLO Lamin A/C control siRNA
siGLO Lamin A/C control (human; 50nM) was transfected into HeLa cells with DharmaFECT 1 (0.15 µg/100µL well). After 48 hours, cells were washed with 1x PBS and stained with Hoechst 3342 nuclear dye (blue).
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.