TOX Transfection Control provides a simple method for assessing transfection efficiency and is cytotoxic only when productively delivered into cells. Cells successfully transfected with the TOX Transfection Control typically undergo apoptosis and cell death within 24 to 48 hours.
This control is partcularly suited for high-throughput siRNA screening when a rapid visual inspection is useful to qualitatively confirm that transfection was successful.
The TOX Transfection control, like other cell death controls, has demonstrated variation in the degree of cell death across different cell types. The efficacy of the control likely requires intact cellular mechanisms, including the apoptosis and interferon pathways. As with any RNAi control, assessment of efficacy in your particular cell type and assay conditions is recommended prior to larger scale use.
Concentrated buffer solution recommended for resuspension and long-term storage of any short, double-strand, or single-strand synthetic RNA molecule. Dilute with RNase-free water prior to use.
Molecular grade water for dilution of 5x siRNA Buffer or resuspension of RNA. RNase-free to prevent degradation of RNA reagents and oligonucleotides.
The most broadly applicable DharmaFECT formulation for optimal siRNA or microRNA transfection into a wide range of cell types for successful RNAi experiments