Luciferase GL2 Duplex
When the Luciferase GL2 Duplex is co-transfected with the reporter plasmid pGL2 (Promega) into mammalian cells, this siRNA control efficiently reduces GL2 expression.
Because the luciferase reporter sequence is often altered to improve its performance, we recommend aligning the siRNA target sequence to your reporter to ensure a sequence match.
Highlights
- Target Sequence:
5'- CGT ACG CGG AAT ACT TCG A -3' - Appropriate for use in any mammalian cell type
- S. M. Elbashir et al., Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411(6836), 494-498 (24 May 2001).
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