Luciferase GL3 Duplex
When co-transfected with the reporter plasmid pGL3 (Promega) into mammalian cells, the Luciferase GL3 Duplex control siRNA effectively reduces GL3 expression.
Because the luciferase reporter sequence is often altered to improve its performance, we recommend aligning the siRNA target sequence to your reporter to ensure a sequence match.
Highlights
- Target Sequence:
5'- CTT ACG CTG AGT ACT TCG A -3' - Appropriate for use in any mammalian cell type
- S. M. Elbashir et al., Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411(6836), 494-498 (24 May 2001).
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