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Mimix™ Geni™ Negative FFPE標準サンプル
Mimix™ Geni™ Negative FFPE 標準サンプルは、Mimix Geni Onco Mix 1 DNA/RNA FFPE 標準サンプル (HD852)に対応するネガティブコントロールです。本製品は、Medical Device Innovation Consortium（MDIC）が主導する体細胞リファレンスサンプル（Somatic Reference Samples: SRS）イニシアチブの一環として、MDICとの共同開発により作製されました。
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Mimix Geni Negative FFPE標準サンプルは、最も広く検証・使用されている細胞株の一つである Genome in a Bottle（GIAB）コンソーシアム由来の親株 GM24385 を用いて作製されています。本製品は、MDICとの共同開発により作製された Mimix Geni Onco Mix 1 DNA/RNA FFPE標準サンプル (HD852) に対する、ワイルドタイプまたは対応するネガティブコントロールとして使用可能です。また、一般的なFFPEワークフロー管理としてもご利用いただけます。これにより、DNA抽出から解析までのエンドツーエンドのワークフローを包括的に検証することが可能となります。
主な特長
- GIAB GM24385細胞株を使用
- HD852に組み込まれた7種類の変異について ネガティブであることを確認済み
- 患者サンプルを模倣した FFPEフォーマットで提供、ワークフロー全体の検証に最適
- HD852と組み合わせた希釈系列を作製することで、分子アッセイの検出限界（LoD）の評価に最適
- HD852に対するネガティブコントロールとして使用可能
シーケンスデータおよび細胞株に関する公開情報をGenome in a Bottleで閲覧可能
Technical Product Information
Only DNA or RNA should be extracted at any one time. Using dual extraction kits is not recommended for this product.
Format: FFPE
Fixation method: 4% PFA (w/v)
Section size: 15µm
Cell density: 150 x 106 cells per block. Approx. 4.5 x 105 cells per section
Expected DNA yield: ≥400ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit
Expected RNA yield: ≥100ng using Chemagic ™ CMG-1212
Genes covered: ERBB2, BRAF, EGFR, PDGFRA, FGFR3, TPM3-NTRK1 fusion, CCDC6-RET fusion.
Allelic frequencies: 0.00% for ddPCR-verified variants
Verified mutations:
| Chromosome | Gene | Variant | Variant Type | Expected Allelic Frequency (%) |
|---|---|---|---|---|
| Chr17 | ERBB2 | V659E | MNV | 0.00% |
| Chr7 | BRAF | V600E | SNV | 0.00% |
| Chr7 | EGFR | A763_Y764insFQEA | INDEL | 0.00% |
| Chr4 | PDGFRA | I843del/ I843_D846del | INDEL | 0.00% |
| Chr4 | FGFR3 | S249C | SNV | 0.00% |
| Chr1 | TPM3-NTRK1 | Fusion | Fusion | 0.00% |
| Chr10 | CCDC6-RET | Fusion | Fusion | 0.00% |
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Quality control
Allelic frequency: Droplet Digital™ PCR
DNA Quantification: Quantifluor®
DNA Integrity: Agarose Gel electrophoresis
RNA Quantification: Qubit® RNA HS Assay (Thermo Fisher Scientific)
RNA Integrity: DV200 ≥65% using TapeStation High Sensitivity RNA ScreenTape Assay (Agilent)
Manufacturing Quality: ISO 13485
Intended Use: For research use only. Not for use in diagnostic procedures.
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Safety data sheets
Selection guides
Reference Standards
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At what temperature do you ship reference standards?
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Can both DNA and RNA be extracted from the same FFPE curl?
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How can I open the NGS data .gz files?
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How do you manufacture your Horizon Reference Standards?
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How do you recommend extracting DNA from an FFPE reference standard?
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How was the batch-specific supplementary NGS data produced and analysed?
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Is it possible to order custom versions of Mimix Reference Standards?
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The FFPE yield is lower than expected, how can I improve the yield?
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What batch-specific supplementary NGS data is provided?
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Where can I access batch-specific supplementary NGS data?
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Which extraction kits have been tested with FFPE RNA reference standards?
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Why do I see different allele frequencies or different variants in my NGS data when comparing to the data provided with the reference standard?