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Edit-R HDR plasmid donor components
Insert and backbone for HDR plasmid construction
The Edit-R HDR Plasmid Donor Kit for insertion of EGFP or mKate2 using the HDR pathway is constructed from a universal plasmid backbone, the DNA insert, and two custom homology arms. Assembly of a custom insert plasmid donor utilizes the same strategy only substituting a user-provided DNA fragment for the insert sequence. These products include the EGFP or mKate2 insert and the plasmid backbone only.
Use and assembly of the donor plasmid requires design of two primer pairs for amplification of the 5′ and 3′ homology arms flanking the insertion site. These primers can be designed and ordered using the HDR Donor Designer.
Highlights
- Fast and efficient assembly of the DNA donor plasmid
- Validated protocols for design, assembly, and confirmation of the donor plasmid
- Utilize the CRISPR Design Tool for designing the crRNA to the cut site
- Seamless integration with the HDR Donor Designer for designing the primers to create the required 5′ and 3′ homology arms flanking the insertion site
Included Components & Amounts
| Catalog Number | Description | Item Number | Quantity |
|---|---|---|---|
| UK-008100-02-10 | Edit-R HDR Plasmid Donor & Insert - EGFP | ||
| Edit-R HDR Plasmid Donor Backbone | U-008300-01-1200 | 1200 ng | |
| Edit-R HDR insert - EGFP | U-008100-01-250 | 250 ng | |
| UK-008200-04-10 | Edit-RHDR Plasmid Donor & Insert -mKate2 | ||
| Edit-RHDR Plasmid Donor Backbone | U-008300-01-1200 | 1200 ng | |
| Edit-RHDR Insert - mKate2 | U-008200-01-250 | 250 ng | |
| U-008300-01-1200 | Edit-R HDR Plasmid Donor Backbone | U-008300-01-1200 | 1200 ng |
Note: The backbone and EGFP or mKate2 inserts are included with the HDR Plasmid Donor Kit; they may be ordered separately if more material is needed.
