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Edit-R lentiviral sgRNA positive controls and kits
Control lentiviral sgRNAs to verify DNA double-strand breaks and gene editing efficiencies.
Validated Edit-R Lentiviral sgRNA Positive Controls and kits confirm generation of insertions and deletions (indels), and permit quantification of gene editing efficiency using DNA mismatch detection assays.
Validated Lentiviral sgRNA Positive Controls and Kits for the verification of gene editing events
Edit-R Lentiviral sgRNA Positive Controls and kits are designed to target human or mouse genes and can be included in all gene editing experiments to confirm successful transduction by observing the generation of insertions and deletions (indels) in your targeted genomic DNA using simple DNA mismatch detection assays.
Individual Edit-R Lentiviral sgRNA Positive Controls are available as glycerol stocks (mouse designs only) and as concentrated, purified lentiviral particles (50 µL at ≥ 1 x 108 TU/mL); available for human or mouse and designed against:- Cyclophilin B (PPIB)
- DNA (cytosine-5)-methyltransferase 3B (DNMT3B)
Edit-R Lentiviral sgRNA Positive Control Kits include:
- Lentiviral particles or glycerol stock (mouse designs only)
- DNA mismatch detection primers (forward and reverse)
The Edit-R Lentiviral CRISPR-Cas9 Gene Engineering platform includes two critical components based on the natural S. pyogenes system: a vector for Cas9 expression and a gene-specific vector for sgRNA expression designed to the target site of interest. To facilitate rapid generation of cells lines that constitutively express Cas9 nuclease and the sgRNA, the Cas9 and sgRNA vectors are available pre-packaged into lentiviral particles that have been purified and concentrated. Cas9-expressing cells lines can be easily generated with the Edit-R Lentiviral Cas9 Nuclease Expression particles and subsequent gene knockouts can be obtained by an additional transduction with sgRNA lentiviral particles.
