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miRIDIAN microRNA Hairpin Inhibitor Positive Control
A validated microRNA inhibitor designed to target miR-16 in human, mouse and rat
The miRIDIAN microRNA Hairpin Inhibitor Positive Control is ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for loss-of-function microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize inhibition experimental conditions by assessing the effect of inhibition on an endogenously expressed microRNA, we offer a validated Hairpin Inhibitor Positive Control.
Highlights
- Targets endogenous miR-16 in human, mouse, and rat cells resulting in reduced miR-16 levels (seeFigure 1, Supporting Data)
- Control designs and modifications are identical to experimental miRIDIAN microRNA Hairpin Inhibitors
Applications
- Optimize microRNA inhibition assays by targeting a microRNA in a cell line that expresses miR-16
- Specific inhibition of miR-16 can be directly assessed by RT-qPCR and/or Northern blotting
