siGENOME Non-Targeting Control siRNAs are highly functional, chemically synthesized, and mostly unmodified negative control siRNA duplexes for experiments in human, mouse, and rat cells. siGENOME Non-Targeting Controls were designed to have a minimum of 4 mismatches to all human, mouse and rat genes, and confirmed to have minimal targeting by genomewide microarray analysis. Changes in mRNA or protein levels in cells treated with these controls reflect a baseline cellular response that can be compared to the levels in cells treated with target-specific siRNA.

Highlights

• BLAST analysis confirms at least 4 mismatches with all known human, mouse, and rat genes
• Screened by microarray analysis

Experimental Considerations

• siGENOME Non-targeting siRNA #1 reduces EGFR mRNA (NM_005228) in an assay- and cell-specific manner by ~50%. Originally reported by customers, this finding was not observed by initial microarray validation, but has been subsequently verified by internal scientists. Recommended only if you have successfully used this control in past experiments
• siGENOME Non-Targeting siRNA #2 and #4 target firefly luciferase mRNA (U47296). These siRNAs can be used either as a non-targeting siRNA in human, mouse or rat cell lines or as a positive silencing control in systems using firefly luciferase as a reporter gene (pGL3 cloning vector, Promega).
• siGENOME Non-Targeting siRNA #5 carries the ON-TARGET modification pattern to prevent sense strand uptake.