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shMIMIC Inducible Lentiviral microRNA
shMIMIC Inducible Lentiviral microRNA
1Start Here
shMIMIC lentiviral microRNA designs are created for the robust over-expression of each mature human, mouse and rat microRNA in the miRBase database while minimizing the effects of the star (*), or passenger, strand. Inducible control of the shMIMIC microRNA and the reporter gene are conferred by the Tet 3G promoter (PTRE3G), while the expression of the Tet-On® 3G transactivator protein is under the control of a constitutive RNA pol II promoter.
The SMARTchoice inducible vector customization options include four inducible promoters (mCMV, PGK, hEF1α and mEF1α) and two reporter (TurboGFP and TurboRFP) options. shMIMIC Inducible Lentiviral microRNA designs are cloned and packaged into lentiviral particles on demand and provided as glycerol stocks and high-titer (≥ 1x107 TU/mL) viral particles.
Highlights
- Unique, patented expression scaffold for consistent and correct processing of the mature microRNA
- Universal primary context ensures that each mature microRNA is correctly and consistently processed
- Design modifications promote preferential loading of the mature microRNA into RISC and robust function
- The constructs are designed to preserve the function of the miRNA repression but may deviate slightly from the mirBase sequence
- Tight control of shMIMIC microRNA and reporter gene expression utilizing the latest generation Tet-inducible expression technology, the Tet-On® 3G Inducible System
- Unsurpassed flexibility conferred by the SMARTchoice suite of vector options maximizes success in a broad spectrum of cell types and applications
- High-titer, concentrated lentiviral particles permit delivery into cells refractory to lipid-based delivery
- Glycerol stocks act as a renewable resource for future experiments
Lentiviral Particle Product Format, Shipping and Storage Information
- shMIMIC Inducible Lentiviral microRNAs are made-to-order with your choice of promoter and reporter. Choose between 100 µL and 200 µL volumes, enough purified and concentrated lentiviral particles to perform high-confidence experiments with biological replicates in difficult-to-transfect cells.
- Lentiviral particles require an estimated 4- to 6-week manufacturing time. Additional transit time should be expected for international shipment to account for customs and regulatory clearances and overseas transport.
- shMIMIC lentiviral particles are shipped on dry ice for overnight domestic delivery or priority international delivery outside of the USA. shMIMIC lentiviral particles can be stored frozen at -80°C for 12 months without a significant loss in titer.
