To order gene-specific SMARTvector Inducible Lentiviral shRNAs or shMIMIC Inducible Lentiviral microRNAs search for your gene of interest and make your desired promoter and reporter choices.

Matched SMARTvector Inducible Negative and Positive RNAi Controls enable accurate data interpretation for both SMARTvector Inducible shRNA and shMIMIC Inducible Lentiviral microRNA

The SMARTvector Inducible RNAi Controls are cost-effective tools for determining optimal conditions for delivery and doxycycline induction, prior to using gene-specific SMARTvector Inducible Lentiviral shRNAs and shMIMIC Inducible Lentiviral microRNAs.

Start by identifying the optimal vector configuration for your cells with the SMARTchoice Inducible Non-targeting Control 4-Pack

Determine the most active promoter in the specific cells of interest by evaluating all four vectors simultaneously using the SMARTchoice Inducible Non-targeting Control 4-Pack (catalog # VSC6847):

• Four aliquots (25 µL each) of SMARTvector Inducible Non-targeting Controls supplied as high-titer, concentrated and purified lentiviral particles
• Includes each SMARTvector Inducible Non-targeting Control with PGK, mouse CMV, human EF1α and mouse EF1α promoters; and with the turboGFP fluorescent reporter
• Enables qualitative, empirical evaluation of optimal promoter driving expression of puromycin resistance and Tet-On 3G transactivator protein

Order SMARTvector inducible RNAi controls with promoter and reporter selections matching your gene-specific constructs.

SMARTvector Inducible vector-matched controls are available with options for all four promoters and two fluorescent reporters or without a fluorescent reporter. All individual controls are available as glycerol stocks and as 50 µL (2 x 25 µL) at 1 x 10⁷ TU/mL, ± 20%, of purified, concentrated packaged lentiviral particles.

SMARTvector Inducible Non-targeting Controls

• Designed so that no known gene in human, mouse or rat is targeted, providing a baseline to which gene-specific effects can be normalized
• Useful for assessing transduction efficiency and the effects of transduction on the cells of interest

Experimental considerations:

• SMARTvector Inducible Non-targeting Control 1: Our longest standing and most commonly used SMARTvector non-targeting control, ideal for supporting most experiments involving only a few genes
• SMARTvector Inducible Non-targeting Control 2 – 12: Designed specifically with the needs of screeners in mind, who may require using several different non-targeting control sequences in follow-up experiments; should be selected if multiple controls are desired
  
  

SMARTvector Inducible Positive Controls

• Permit confirmation of transduction efficiency and gene knockdown through consistent, potent silencing of non-essential, abundantly expressed housekeeping genes
• SMARTvector Inducible GAPD Positive Control shRNA (human, mouse and rat); validated to target glyceraldehyde-3-phosphate dehydrogenase (also known as GAPDH)
• SMARTvector Inducible PPIB Positive Control shRNA (human, mouse and rat); validated to target peptidylprolyl isomerase B (also known as cyclophilin B)