The RNAi consortium (TRC) is a collaborative effort based at the Broad Institute of MIT and Harvard, and includes six MIT- and Harvard-associated research institutions and five international life sciences organizations. We have partnered with the TRC to make the shRNA libraries available to researchers worldwide. Available TRC shRNA controls include the pLKO.1 empty vector as a negative control and an eGFP shRNA as a positive control.

The pLKO.1 controls can be packaged into virus particles for use in transduction experiments. Specifically, the replication-incompetent viral particles can be efficiently produced using lentiviral packaging plasmids co-transfected in 293T packaging cells. The Trans-Lentiviral packaging system, which offers maximum biosafety and high titers, is recommended.

Highlights

• Rules-based shRNA design for efficient gene knockdown
• Already cloned into lentiviral vectors
• Amenable to in vitro and in vivo applications such as the creation of stable knockdowns
• Lentiviral vector enables transduction of primary and non-dividing cell lines
• Broad coverage: 4 to 5 constructs per gene

Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html

Citing the library in Scientific Publications

In scientific publications, the libraries should be referred to as TRC-Hs1.0 (Human) and TRC-Mm1.0 (Mouse). Individual clones are uniquely identified by their TRC ID number (eg. TRCN0000014783).

The TRC lentiviral shRNA libraries are provided in 96-well microtiter plates containing frozen stock cultures of E. coli in 2X LB broth with 8% glycerol and carbenicillin (100 µg/ml). We check all cultures for growth prior to shipment. Individual shRNA constructs are shipped as glycerol stock cultures on wet ice.