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CRISPRmod CRISPRa lentiviral sgRNA non-targeting controls
Lentiviral sgRNA constructs bioinformatically designed and validated to not target any gene in human or mouse genomes
CRISPRa lentiviral sgRNA non-targeting controls are recommended as negative controls for experiments using CRISPRa lentiviral sgRNAs in human and mouse cells. They are designed to have a minimum of three mismatches or gaps to all potential PAM-adjacent targets in human, mouse and rat genomes. When using these controls, changes in cellular viability or gene expression likely reflect nonspecific cellular responses that can be used as a baseline for comparison to cells treated with target-specific reagents.
Highlights
- CRISPRa guide RNA non-targeting control sequences are cloned into the same optimized lentiviral vector as CRISPRa gene targeting sgRNAs
- Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in human, mouse or rat genomes
- Available as high-titer purified lentiviral particles or glycerol stocks
