Optimal shRNA expression for high-confidence functional analysis results

Our most advanced shRNA platform, SMARTvector lentiviral shRNAs utilize the flexibility of promoter and reporter options coupled with our most advanced rational design algorithm to target the complete genome for human and mouse protein-coding and long non-coding genes.

Pooled lentiviral libraries offer an efficient and cost-effective method for screening large numbers of genes without automation. Library construction, pooling techniques, and high-throughput sequencing-compatible screening workflows have been experimentally validated to ensure reproducibility and accurate hit identification. Learn about optimal library design and the pooled screening workflow on our Pooled lentiviral screening libraries page.

Highlights:

• Innovative vector design for robust shRNA expression from SMARTvector Lentiviral shRNAs
• SMARTchoice options give you a choice of promoter* and reporter (or no reporter!) for optimal performance in your experiment.
• Rationally designed shRNAs for maximum knockdown and minimal off-targeting

Choose SMARTvector inducible lentiviral shRNA for tight regulation of expression from the Tet-On® 3G transactivator protein

*Some promoter options may only be available as custom reagents or upon request.

Each SMARTvector lentiviral shRNA library contains

• ≥ 5 x 10⁸ TU/mL (+/- 20 %) lentiviral particles provided in pre-aliquoted tubes
• 8 x 25 µL (200 µL total) for pools ≤ 5000 shRNA constructs
• 16 x 25 µL (400 µL total) for pools > 5000 shRNA constructs
• 100 Non-targeting control constructs
• 272 constructs targeting 34 human or mouse protein coding genes as gene-specific controls (8 shRNA per gene); includes common reference genes (PPIB, GAPD, Actin, Lamin) and viability-related genes.
• A data file containing construct sequences, target gene IDs, and counts per millions of mapped reads

SMARTvector lentiviral shRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; custom SMARTvector lentiviral shRNA collections are available upon request.

• Request a custom collection of human or mouse gene-targeting shRNAs
• Available in pool sizes between 50 to 10,000 constructs
• Pools are provided as purified, concentrated lentiviral particles
• ≥ 5 x 10⁸ TU/mL for constitutive vectors and ≥ 5 x 10⁷ TU/mL for inducible vectors

Request pricing for a custom pooled library

Before ordering, download these invaluable tools to carefully plan your pooled lentiviral shRNA screen and calculate the amounts of components required:

• SMARTvector & shMIMIC lentiviral pooled libraries technical manual
• Pooled Lentiviral Screening Laboratory Protocols & Calculation Tracking Worksheet

Reagents recommended in our validated protocol:

• Vector-matched SMARTvector non-targeting control for transduction optimization
• SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for:
  • Efficient PCR amplification of genomic DNA with minimal bias
  • High-throughput multiplexed Next Gen Sequencing for hit identification

SMARTvector lentiviral shRNA pooled library coverage*:

Important Notice

The SMARTvector lentiviral shRNA pooled libraries are solely for internal research use (as set forth in the Product Terms and Conditions) in laboratories where the containment measures stated below and in applicable laws and regulations are met. Reagents may not be used for diagnostic, therapeutic or other commercial purposes and may not to be administered to humans for any purpose or to animals for therapeutic purposes. SMARTvector lentiviral shRNA pooled libraries are provided as lentiviral particles are replication-incompetent, self-inactivating (SIN) and non-pathogenic (do not cause infectious human disease).

Any investigator who purchases lentiviral particle reagents is responsible for consulting with their institution's health and biosafety personnel for specific guidelines on the handling of lentiviral vector particles. Furthermore, each investigator is fully responsible for obtaining the required permissions for research using and the acceptance of replication-incompetent SIN lentiviral vectors and replication-defective lentiviral particles into their local jurisdiction and institution.