The best way to figure out what experimental conditions will result in high efficiency CRISPR-Cas9 gene editing in your cells is SIMPLE - use a positive control! A synthetic crRNA positive control will enable you to work out key experimental variables such as transfection, concentration and timing parameters.
Synthetic crRNA Positive Controls and DNA mismatch detection primers
Ensure your experimental conditions are ideal for reproducible, efficient gene editing. We have validated positive controls for the cyclophilin B and DNMT3B genes. We also provide forward and reverse DNA primers designed to amplify across the targeted area for verification of your results with a mismatch detection assay. In short, we give you everything you need to optimize your CRISPR-Cas9 gene editing conditions!
Additional Resources
-
CRISPR Controls and Detection Primers
Ensure your system is optimized for successful CRISPR-Cas9 experiments -
CRISPR Design Tool
Generate custom synthetic crRNA sequences to knockout genes -
Edit-R Lentiviral Cas9 Nuclease Reagents
Find the best Cas9 nuclease expression vector for your cells