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Edit-R Synthetic sgRNA T-cell Receptor Targets

Constant regions of the T-cell receptor for the following targets: TRAC, TRBC, TRDC

Validated Edit-R guide RNAs targeting T Cell Receptor genes

T Cell Receptor genes are made up of variable and constant regions that undergo rearrangement at the genomic level during T cell development to create a vast repertoire of receptors. These receptors are critical for the recognition of foreign antigens and the subsequent immune response. The complexity of these genomic rearrangements makes CRISPR guide RNA design challenging in these regions. We have used our decades of experience with algorithm development and RNA design to overcome these design challenges to successfully create and validate guide RNAs that target and efficiently knockout the T cell receptors.

We validated the guides showing a >90% TCR knockout efficiency using RNP-based editing in primary CD4+ T cells, CD8+ T cells, and induced pluripotent stem cells (iPSCs) and functionally characterized CD8+ CAR-T cells after TCR α/β knockout. Read the High-efficiency endogenous TCR knock-out and functional validation of CD8+ CAR-T cells - App Note to learn more. 

TCR Formation Schematic

TRAC (GeneID: 28755; TCRA, IMD7, TRCA, TRA, TCRA, T cell receptor alpha constant): T cell receptors recognize foreign antigen that have been processed into peptides and bound to MHC molecules at the surface of antigen presenting cells. After rearrangement of the V(variable)-D(diversity)-J(joining) segments at the DNA level, T cell receptor alpha chain constant (TRAC) region is spliced to the V-D-J segment of the T cell receptor alpha chain at the RNA level.

TRBC1/TRBC2 (GeneID: 6957; TRB, TCRB, T cell receptor beta locus/cluster): T cell receptors recognize foreign antigen that have been processed into peptides and bound to MHC molecules at the surface of antigen presenting cells. After rearrangement of the V(variable)-D(diversity)-J(joining) segments at the DNA level, T cell receptor beta chain constant (TRBC) region is spliced to the V-D-J segment of the T cell receptor beta chain at the RNA level.

TRDC (GeneID: 28256; TRDC, T cell receptor delta constant): T cell receptors recognize foreign antigen that have been processed into peptides and bound to MHC molecules at the surface of antigen presenting cells. After rearrangement of the V(variable)-D(diversity)-J(joining) segments at the DNA level, T cell receptor delta chain constant (TRDC) region is spliced to the V-D-J segment of the T cell receptor delta chain at the RNA level.