CRISPR Design Tool Workflow

Online tool to design and order custom guide RNAs

Design and order custom guide RNAs for targeted gene knockout or HDR-mediated genome editing.

  • Rigorous specificity checking identifies designs with potential off-targets
  • Target protein coding, microRNA, or lncRNA genes for over 40 species
  • Synthetic guide RNAs provided in tubes or plates, lentiviral sgRNA as glycerol stock or concentrated lentiviral particles
  • Advanced options enable transcript-specific designs or designs for a novel sequence
  • Design guide RNA for S. pyogenes Cas9, MAD7, and other CRISPR-Cas systems

The flexibility of the CRISPR Design Tool provides an intuitive one-stop location for custom guide RNA design and ordering.

Need to design a guide RNA?
  • Choose from over 40 model organisms
  • Support for protein coding, long noncoding, or microRNA genes; simply enter a gene identifier!
  • Design guide RNA for S. pyogenes Cas9, MAD7 (Cas12a), or any other CRISPR effector
  • Target a specific region for optimal HDR knock-in studies
  • Unparalleled specificity checking for reduced off-target cleavage
  • Intuitive interface lets you visualize guide RNA designs along your gene
Already have a guide RNA design?
  • Enter a target sequence(s) to easily order lentiviral or synthetic guide RNAs for use with S. pyogenes Cas9 nuclease
  • Enter guide RNA sequences for CRISPR-Cas systems such as MAD7, Cas12a, Cas13a, or completely novel systems
Looking for functional knockout without a design step?

You may also choose from highly functional predesigned synthetic crRNA or lentiviral sgRNA to knockout your human or mouse gene target. Simply search for your gene.