Online tool to design and order custom guide RNAs
Design and order custom guide RNAs for targeted gene knockout or HDR-mediated genome editing.
- Rigorous specificity checking identifies designs with potential off-targets
- Target protein coding, microRNA, or lncRNA genes for over 40 species
- Synthetic guide RNAs provided in tubes or plates, lentiviral sgRNA as glycerol stock or concentrated lentiviral particles
- Advanced options enable transcript-specific designs or designs for a novel sequence
- Design guide RNA for S. pyogenes Cas9, MAD7, and other CRISPR-Cas systems
The flexibility of the CRISPR Design Tool provides an intuitive one-stop location for custom guide RNA design and ordering.
Need to design a guide RNA?
- Choose from over 40 model organisms
- Support for protein coding, long noncoding, or microRNA genes; simply enter a gene identifier!
- Design guide RNA for S. pyogenes Cas9, MAD7 (Cas12a), or any other CRISPR effector
- Target a specific region for optimal HDR knock-in studies
- Unparalleled specificity checking for reduced off-target cleavage
- Intuitive interface lets you visualize guide RNA designs along your gene
Already have a guide RNA design?
- Enter a target sequence(s) to easily order lentiviral or synthetic guide RNAs for use with S. pyogenes Cas9 nuclease
- Enter guide RNA sequences for CRISPR-Cas systems such as MAD7, Cas12a, Cas13a, or completely novel systems
Looking for functional knockout without a design step?
You may also choose from highly functional predesigned synthetic crRNA or lentiviral sgRNA to knockout your human or mouse gene target. Simply search for your gene.