Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 20%, and may be diluted using the corresponding wild type standard to generate a lower allelic frequencies for limit of detection studies and validation of your pre-analytical phase of the workflow. Horizon’s Base-Seq products are all derived from human cell lines. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly-reproducible and consistent FFPE curls. With any commercially available FFPE extraction protocol, our standards yield high molecular weight DNA. This format ensures that they may applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

• Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
• Analyze the sensitivity of your workflow
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Technical Data

Synonyms
B-Raf, BRAF1

Format
FFPE

DNA Base Change

GTG→GAG

NCBI Reference Assembly SNP

rs113488022

Cosmic ID

COSM476

Fixation Method

4% Formalin

Section Size

15µm or 20µm

Cell Density

3 x 10⁸ cells per block. Approx. 3.5 x 105 cells per section

Expected DNA Yield

≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

Product Information

Intended Use

For routine performance monitoring (Research Use Only)

Unit Size

1 FFPE Section per vial

Extractable DNA

≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

General Information

Allelic Frequency

20%

Storage

4˚C

Expiry

See all product shelf life information

Cell Line Background

HCT116

Quality Control

Allelic Frequency

Droplet Digital PCR™

Genotype

Sanger sequencing of locus specific PCR

Quality

Agarose gel electrophoresis

Quantification

Quantifluor™

Genotype

BRAF (V600E/+)

Characterization

Chromatogram

Chromatograph showing heterozygosity for the B-Raf V600E mutation within B-Raf exon 15 (SNP accession number: rs113488022)

Primers