Mimix™ BRCA Somatic Multiplex I, gDNA Reference Standard

The BRCA Somatic Multiplex Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations.

For a limited time only, buy any 2 of the same Mimix reference standard products and get the 3rd FREE!*

*This promotion is only valid in China from 1st July 2025 to 30th September 2025 and on products of the same part number. The promotion cannot be combined with any other promotions, discounts, or offers. Conditions of the promotion may change, and Revvity may void, change, cancel, suspend, or amend this promotion without notice.

BRCA Somatic Multiplex Reference Standard Genomic DNA

With this product you are able to:

  • Analyze the specificity and specificity of your assay
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

 

Technical Data

Format: Genomic DNA

Genes Covered: BRCA1, BRCA2, BRIP1, BARD1 and NBN

Allelic Frequencies: 7.5-60%

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

 

Product Information

Verified Mutations: The BRCA Somatic Multiplex I Reference Standard has been manufactured using multiple engineered cell lines and mixed to generate multiple Allelic Frequencies in multiplex.

Gene Variant (AA) CDS mutation GRCh38 coordinates Expected allelic frequency (%) NGS allelic frequency (%) NGS read depth
BARD1 R378S c.1134G>C 2:214780740 42.50% 40.79% 885
BRCA1 D435Y c.1303G>T 17:43094228 7.50% 7.16% 810
BRCA1 K1183R c.3548A>G 17:43091983 7.50% 7.98% 815
BRCA1 K820E c.2458A>G 17:43093073 7.50% 8.76% 1336
BRCA1 P871L c.2612C>T 17:43092919 15.00% 15.02% 1052
BRCA1 R1443* c.4327C>T 17:43082434 32.50% 32.84% 886
BRCA1 S1634G** c.4900A>G 17:43071077 7.50% 7.51% 1145
BRCA2 D1420Y c.4258G>T 13:32338613 32.50% 31.31% 907
BRCA2 I2675Dfs*6 c.8021dup 13:32363217 10.00% 8.29% 603
BRCA2 K1691Nfs*15 c.5073del 13:32339421 32.50% 32.12% 909
BRCA2 N1784Tfs*7 c.5351del 13:32339699 40.00% 36.99% 1011
BRCA2 N289H c.865A>C 13:32332343 7.50% 7.23% 996
BRCA2 N991D c.2971A>G 13:32337326 7.50% 6.63% 784
BRCA2 V2466A c.7397T>C 13:32355250 100.00% 100.00% 717
BRIP1 S919P c.2755T>C 17:61685986 100.00% 99.88% 801
NBN E185Q c.553G>C 8:89978251 60.00% 64.62% 636

** This variant is also known by its secondary transcript: p.S1613G/c.4837A>G. We may use this interchangeably across our website and resources.

Unit Size: 1µg

Concentration: 50ng/µl

Exome sequencing data: Download additional information on all exonic variants present in this product. Please note: this NGS data is not batch specific.

 

General Information

Storage: 4˚C

Expiry: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260)

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.