Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard may be used to dilute corresponding variant standard to generate lower allelic frequencies for limit of detection studies.Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

• Analyze the sensitivity of your assay
• Gain certainty of the limit of detection
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Technical Data

Synonyms: IDH, IDP, PICD

Format: Genomic DNA

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.37

Product Information

Intended Use: For routine performance monitoring (Research Use Only)

Unit Size: 5µg

Concentration: 50ng/µl

General Information

Allelic Frequency: WT

Storage: 4˚C

Expiry: See all product shelf life information

Cell Line Background: HCT116

Exome sequencing data: Download additional information on variants present in the parental cell lines

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260)

Genotype: IDh2 (+/+)

Characterization

Gel Image:

DNA was run on a 1% agarose gel and shows a single high-molecular weight band

Chromatogram:

Chromatograph showing heterozygosity for the IDh4 R140Q mutant sample and homozygous normal for the IDh4 wild type

Primers: