KRAS G12A Reference Standard, 50% 1 FFPE curl
The KRAS G12A Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.
Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.
Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.
Product Description: KRAS G12A Reference Standard, 50% 1 FFPE curl
Catalog number: HD255
Amino Acid Change: G12A
Nucleotide change: c.35G>C
Variant Allele Frequency: 50%
Cosmic Genomic Mutation ID: COSV55497479
Cosmic Legacy ID: COSM522
GRCh38 position: 12:25245350
Unit Size: 15 µm section
Fixation Method: 4% PFA (w/v)
Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit
Expiry: See all product shelf-life information
Cell Line Background: SW48
Exome Sequencing Data: Download additional sequencing data from the parental cell
Genotype and Allelic Frequency: Droplet Digital PCRTM
DNA integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 13485:2016
Use: Research Use Only
Cell Line Characterization
Chromatograph showing heterozygosity for the K-Ras G12A mutation within K-Ras exon 2 (SNP accession number: rs121913529)
Size / bp
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