Our website has a fresh new "look"

We've updated our site with modern design and easier navigation, but you'll still find the same quality Dharmacon™ reagents and Mimix™ reference standards that you love.

We hope you are enjoying the modern design and navigation of our website. Have suggestions for us? Share feedback here.

Mimix™ KRAS G12D 50%, gDNA Reference Standard

The KRAS G12D Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

Special offer! For a limited time only, we’re offering a special discounted price on selected gDNA products. Available while stocks last.*

*This pricing promotion is only valid on selected products and while stocks last. Not valid in Italy, China, and Japan. The promotion cannot be combined with any other promotions, discounts, or offers. Conditions of the promotions may change, and Revvity may void, change, cancel, suspend, or amend this promotion without notice.

Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies. Our products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

  • Analyze the sensitivity of your assay
  • Gain certainty of the limit of detection
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

 

Technical Data

Synonyms: C-Ki-Ras, K-RAS2B, KI-RAS, KRAS2, RASK2

Format: Genomic DNA

DNA Base Change: GGT→GAT

NCBI Reference Assembly SNP: rs121913529

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

 

Product Information

Unit Size: 1µg

Concentration: 50ng/µl

 

General Information

Allelic Frequency: 50%

Storage: 4˚C

Expiry: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260)

Genotype: KRAS (G12D/+)

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.