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Mimix™ Lymphoid Cancer Panel Negative Control, gDNA Reference Standard

A negative control for additional validation of lymphoma cancer assays

To develop robust molecular and genetic companion diagnostic (CDx) tests, the use of reference material is imperative. Unlike synthetic alternatives, our engineered cancer cell line-based reference standards closely mimic patient samples. The Lymphoid Cancer genomic DNA panel has 10 SNVs and 3 CNVs relevant in major non-Hodgkin lymphomas. And because lymphomas are typically somatic, our panel contains allele frequencies as low as 5%. Further dilution to suit your assay cut-offs is possible with this matched negative control. All mutations are thoroughly validated and manufactured under ISO 13485. 

 

Technical Data

Format: Genomic DNA

Genes Covered: BRAF, CDKN2A, CXCR4, DNMT3A, EZH2, IDH2, MYD88, NRAS, RB1, SOCS1, STAT3, TET2, TP53

Allelic Frequencies: 0%

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

 

Product Information

Verified mutations:

Gene DNA sequence change Amino acid change Expected allelic frequency (%)
BRAF c.1799T>A p.V600E 0.00
CDKN2A c.1-471del471 N/A 2 copies
CXCR4 c.1012C>T p.R338* 0.00
DNMT3A c.2644C>T p.R882C 0.00
EZH2 c.1937A>G p.Y646C 0.00
IDH2 c.514A>G p.R172G 0.00
MYD88 c.818T>C p.L273P 0.00
NRAS c.181C>A p.Q61K 0.00
RB1 c.138_264del127 n/a 2 copies
SOCS1 c.1-636del636 n/a 2 copies
STAT3 c.1981G>T p.D661Y 0.00
TET2 c.679del p.E227fs 0.00
TP53 c.586C>T p.R196* 0.00

 

Unit Size: 500ng

Concentration: 25ng/µl

 

General Information

Storage: 4˚C

 Expiration: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

DNA extraction: Maxwell platform

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260) 

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.