Myeloid Cancer Panel cfDNA Negative Control Reference Standard
A negative control for the Myeloid Cancer Panel cell-free DNA reference standard. Designed specifically to complement the myeloid cancer reference standards, this product is confirmed negative for 15 clinically-relevant variants across 14 key oncogenes.
Technical data
Format: cfDNA
Genes covered: ABL1, ASXL1, CBL, DNMT3A, EZH2, FLT3, IDH1, IDH2, JAK2, NPM1, NRAS, SF3B1, TET2, TP53
Allelic frequencies: 0.00%
Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product information
Fragment size: 160 bp
Unit size: 350 ng per vial
Concentration: 20 ng/µl
Verified mutations:
| Gene | DNA sequence change | Amino acid change | Expected allelic frequency (%) |
|---|---|---|---|
| ABL1 | c.1001C>T | p.T315I | 0.00 |
| ASXL1 | c.2388G>T | p.W796C | 0.00 |
| CBL | c.1208C>T | p.S403F | 0.00 |
| DNMT3A | c.2644C>T | p.R882C | 0.50 |
| EZH2 | c.1253G>A | p.R418Q | 0.00 |
| FLT3 | c.253G>T | p.D835Y | 0.00 |
| IDH1 | c.394C>T | p.R132C | 0.00 |
| IDH2 | c.515G>A | p.R172K | 0.00 |
| JAK2 | c.1611_1616del | p.F537-K539>L | 0.00 |
| JAK2 | c.1849G>T | p.V617F | 0.00 |
| NPM1 | c.860_863dup | p.W288Cfs*12 | 0.00 |
| NRAS | c.182A>T | p.Q61L | 0.00 |
| SF3B1 | c.2219G>T | p.G740E | 0.00 |
| TET2 | c.3782G>A | p.R1261H | 0.00 |
| TP53 | c.722C>T | p.S241F | 0.00 |
General information
Storage: 4°C
Expiration: See all product shelf life information
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit® dsDNA BR assay (Invitrogen)
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Application notes
Certificate of analysis
Related Products
The popular myeloid cancer panel is now available in cell-free DNA to support validation of liquid biopsy-based MRD assays
The Myeloid DNA Reference Standard is a well characterised, cell line derived control material manufactured under ISO 13485 that contains 22 variants across 19 genes relevant to myeloid cancer