Mimix™ Myeloid Cancer Panel cfDNA Negative Control Reference Standard
A negative control for the Myeloid Cancer Panel cell-free DNA reference standard. Designed specifically to complement the myeloid cancer reference standards, this product is confirmed negative for 15 clinically-relevant variants across 14 key oncogenes.
Technical data
Format: cfDNA
Genes covered: ABL1, ASXL1, CBL, DNMT3A, EZH2, FLT3, IDH1, IDH2, JAK2, NPM1, NRAS, SF3B1, TET2, TP53
Allelic frequencies: 0.00%
Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0
Product information
Fragment size: 160 bp
Unit size: 350 ng per vial
Concentration: 20 ng/µl
Verified mutations:
| Gene | DNA sequence change | Amino acid change | Expected allelic frequency (%) |
|---|---|---|---|
| ABL1 | c.1001C>T | p.T315I | 0.00 |
| ASXL1 | c.2388G>T | p.W796C | 0.00 |
| CBL | c.1208C>T | p.S403F | 0.00 |
| DNMT3A | c.2644C>T | p.R882C | 0.50 |
| EZH2 | c.1253G>A | p.R418Q | 0.00 |
| FLT3 | c.253G>T | p.D835Y | 0.00 |
| IDH1 | c.394C>T | p.R132C | 0.00 |
| IDH2 | c.515G>A | p.R172K | 0.00 |
| JAK2 | c.1611_1616del | p.F537-K539>L | 0.00 |
| JAK2 | c.1849G>T | p.V617F | 0.00 |
| NPM1 | c.860_863dup | p.W288Cfs*12 | 0.00 |
| NRAS | c.182A>T | p.Q61L | 0.00 |
| SF3B1 | c.2219G>T | p.G740E | 0.00 |
| TET2 | c.3782G>A | p.R1261H | 0.00 |
| TP53 | c.722C>T | p.S241F | 0.00 |
General information
Storage: 4°C
Expiration: See all product shelf life information
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit® dsDNA BR assay (Invitrogen)
Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.
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If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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Application notes
Related Products
The popular myeloid cancer panel is now available in cell-free DNA to support validation of liquid biopsy-based MRD assays
The Myeloid DNA Reference Standard is a well characterised, cell line derived control material manufactured under ISO 13485 that contains 22 variants across 19 genes relevant to myeloid cancer