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Mimix™ Myeloid Cancer Panel, cfDNA Reference Standard

The popular myeloid cancer panel is now available in cell-free DNA to support validation of liquid biopsy-based MRD assays

The Myeloid Cancer Panel cfDNA reference standard is a well-characterized cell line-derived control which contains 15 clinically-relevant variants across 14 genes. This cell-free DNA reference material allows labs to perform reliable and cost-effective validations of myeloid cancer liquid biopsy assays. QC pipelines are supported by controls manufactured under ISO:13485 with low allele frequencies, designed specifically to replicate cell-free patient samples in minimal residual disease (MRD) and liquid biopsy assays. 

 

Technical data

Format: cfDNA

Genes covered: ABL1, ASXL1, CBL, DNMT3A, EZH2, FLT3, IDH1, IDH2, JAK2, NPM1, NRAS, SF3B1, TET2, TP53

Allelic frequencies: 0.50% - 1.00%

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

 

Product information

Fragment size: 160 bp

Unit size: 350 ng per vial

Concentration: 20 ng/µl

Verified mutations: 

Gene DNA sequence change Amino acid change Expected allelic frequency (%)
ABL1  c.1001C>T  p.T315I  0.50
ASXL1  c.2388G>T  p.W796C  0.50
CBL  c.1208C>T  p.S403F  0.50 
DNMT3A  c.2644C>T  p.R882C  0.50
EZH2  c.1253G>A  p.R418Q  0.50
FLT3  c.253G>T  p.D835Y  0.50
IDH1  c.394C>T  p.R132C  0.50
IDH2  c.515G>A  p.R172K  0.50
JAK2  c.1611_1616del  p.F537-K539>L  0.50
JAK2  c.1849G>T  p.V617F  0.50
NPM1  c.860_863dup  p.W288Cfs*12  0.50
NRAS  c.182A>T  p.Q61L  1.00
SF3B1  c.2219G>T  p.G740E  0.50
TET2  c.3782G>A  p.R1261H  0.50
TP53  c.722C>T  p.S241F  0.50

 

General information

Storage: 4°C

Expiration: See all product shelf life information

 

Quality control

Fragmentation size: D1000 DNA ScreenTape assay

Allelic frequency: Droplet Digital™ PCR

Quantification: Qubit® dsDNA BR assay (Invitrogen)

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.

Related Products

Mimix™ Myeloid Cancer Panel, gDNA Reference Standard

The Myeloid DNA Reference Standard is a well characterised, cell line derived control material manufactured under ISO 13485 that contains 22 variants across 19 genes relevant to myeloid cancer

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Mimix™ Myeloid Cancer Panel Negative Control, cfDNA Reference Standard

A cell-free DNA negative control designed to complement the Myeloid Cancer Panel

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