NRAS Wild Type Reference Standard 5 ug
The NRAS Wild Type Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.
Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 0% for variants of the indicated gene and may be used to dilute corresponding variant standard to generate lower allelic frequencies for limit of detection studies.Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.
With this product you are able to:
- Analyze the sensitivity of your assay
- Gain certainty of the limit of detection
- Optimize and validate new cancer panels and routinely monitor the performance of your assay
Technical Data
SynonymsN-Ras
FormatGenomic DNA
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.64
Product Information
Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
5µg
Concentration
50ng/µl
General Information
Allelic Frequency
100%
Storage
4˚C
Expiry
See all product shelf life information
Cell Line Background
SW48
Quality Control
Allelic Frequency
Droplet Digital PCR™
Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis
Quantification
Spectrophotometry (A260)
Genotype
NRAS (+/+)
Characterization
Gel Image
Chromatogram
Primers
| Primer | Sequence | Size |
| Forward | GCTTATTTAACCTTGGCAATAGCA | 521 |
| Reverse | CCAAGTCATTCCCAGTAGCAA | |
| Sequencing | GCTTATTTAACCTTGGCAATAGCA | -- |