Mimix™ OncoSpan, FFPE Reference Standard
This large panel NGS control contains over 380 variants across more than 152 key cancer genes.
Product name change
As part of the continuous evolution of our portfolio, we have changed the name of our range to Mimix™ reference standards to better reflect the patient-like performance they provide.
While our product range has a new name, everything you rely on remains the same:
- Identical formulation and performance
- Same part numbers and SKUs
- Existing protocols and procedures
- Same technical specifications
- Quality and reliability you trust
No action is required from you. Future orders, product labels, documentation, and communications will reference the new product name.
OncoSpan is a well-characterized, cell line-derived Reference Standard containing over 380 variants across 152 key cancer genes. Variants are present between 1-100% allelic frequency (AF), with 50 variants present at ≤ 20% AF for LOD determination of your assay. Every batch of OncoSpan DNA has 25 variants confirmed by ddPCR, in addition to being fully exome sequenced (Batches before 54952 were sequenced at 500x coverage on an Illumina platform using the Agilent SureSelect Human All Exon V6 kit. Batches from 54952 are sequenced at 500x coverage by Revvity Omics using the Agilent CREV3 kit and an Illumina platform). This provides you with an accurate and reliable truth set for comparison to your assay’s performance. You will receive a password upon ordering to download the raw NGS data for your batch (bam, bed and vcf files). Alternatively, for your convenience we have also listed the 386 high confidence variants in an easy-to-access excel document that can be downloaded.
Technical Data
Format: FFPE
Genes Covered: ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDh2, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDh2, IDh4, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLh2, MLLT3, MMAB, MRE11, MSh4, MSH6, MTOR, NBN, NF1, NFE2L2, NOTCh1, NOTCh4, NOTCh6, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCh2, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395
Allelic Frequencies: 1% - 92.5% for ddPCR-verified variants
Product Information
Verified Mutations:
|
Chromosome |
Gene |
Variant |
Expected Allelic Frequency % |
|
chr2 (29416025) |
ALK |
N/A (Ins) |
10.0% |
|
chr5 (112175770) |
APC |
p.T1493T |
35.0% |
|
chr7 (140453136) |
BRAF |
p.V600E |
10.7% |
|
chr13 (32913558) |
BRCA2 |
p.K1691fs*15 |
32.5% |
|
chr3 (41266101) |
CTNNB1 |
p.S33Y |
32.5% |
|
chr3 (41266133) |
CTNNB1 |
p.S45del |
10.0% |
|
chr7 (55241707) |
EGFR |
p.G719S |
24.5% |
|
chr7 (55249071) |
EGFR |
p.T790M |
0.90% |
|
chr7 (55259515) |
EGFR |
p.L858R |
2.80% |
|
chr7 (55242464) |
EGFR |
p.E746_A750 delELREA |
1.90% |
|
chr7 (55249063) |
EGFR |
p.Q787Q |
15.0% |
|
chr4 (153244155) |
FBXW7 |
p.S668fs*39 |
32.5% |
|
chr13 (28578214) |
FLT3 |
p.P986fs*>8 |
10.0% |
|
chr4 (55599321) |
KIT |
p.D816V |
10.0% |
|
chr4 (55602765) |
KIT |
p.L862L |
7.5% |
|
chr12 (25398281) |
KRAS |
p.G13D |
15.0% |
|
chr12 (25398284) |
KRAS |
p.G12D |
6.30% |
|
chr7 (116436022) |
MET |
p.A1357A |
7.0% |
|
chr7 (116339847) |
MET |
p.L238fs*25 |
7.0% |
|
chr9 (139409754) |
NOTCh1 |
p.P668S |
30.0% |
|
chr1 (115256530) |
NRAS |
p.Q61K |
12.5% |
|
chr3 (178936091) |
PIK3CA |
p.E545K |
8.80% |
|
chr3 (178952085) |
PIK3CA |
p.H1047R |
17.5% |
|
chr10 (43613843) |
RET |
p.L769L |
60.0% |
|
chr17 (7579472) |
TP53 |
p.P72R |
92.5% |
Unit Size: 1 FFPE section
Fixation: 4% PFA
Section Size: 15 µm
Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA yield: >400ng using Maxwell® 16 FFPE plus RSC DNA Purification Kit (Promega)
Storage: 4˚C
Expiration: See all product shelf life information
Quality Control
Allelic Frequency: Droplet Digital PCR™
Genotype: Next Generation Sequencing
Quality: Agarose gel electrophoresis
Quantification: QuantiFluor®
Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.
OncoSpan publications
Read these research articles citing the use of OncoSpan reference standards.
- Validation of a pan-cancer targeted next generation sequencing panel in New Zealand | New Zealand Journal of Medical Laboratory Science
- Enabling variant calling in challenging FFPE samples by coupling a novel library preparation chemistry with exome sequencing | Journal of Clinical Oncology
- Moving towards a local testing solution for undetermined thyroid fine-needle aspirates: validation of a novel custom DNA-based NGS panel | Journal of Clinical Pathology
- Performance Analysis of Three Bioinformatic Variant Callers Using a Somatic Reference Standard | Children's Mercy Kansas City
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Application notes
Product data
Selection guides
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