Human ON-TARGETplus siRNA Library - Ubiquitin Conjugation Subset 3
siRNA designed and modified for greater specificity
The Human ON-TARGETplus Ubiquitin Conjugation Subset 3 siRNA library contains RING finger and RING finger-like E3 Ligases; the large majority of known ubiquitin ligases. RING finger E3 ligases and most of the RING finger-like E3s form a site of interaction with E2s through a platform generated as a consequence of coordination of two zinc ions.
U-box E3s conform like RING finger proteins except that they do so through salt bridges and other interactions that do not involve coordination of zinc. A20-like proteins, also included in this set, represent the newest class of E3 ligases and contain a single coordination site for zinc. This set contains siRNA for well-known E3s, such as BRCA1, MDM2, and CBL family members.
Highlights
- Patented dual-strand ON-TARGETplus modification pattern on all siRNAs to reduce off-targets
- Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
- Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
- Available as SMARTpool siRNA reagents or a Set of 4 siRNAs in 96-well plates
- Guaranteed target gene knockdown (see Specifications tab)
Gene targets
For a complete list of target genes in this siRNA Library, please contact Scientific Support or your local Sales Representative.
Experimental considerations
For a thorough investigation of the ubiquitin pathway, you may also consider these additional libraries:
