miRIDIAN microRNA Mimic Endogenous Positive Control

A validated miRIDIAN microRNA Mimic designed to target Aldolase A in human, mouse, and rat

The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines.
dharmacon reagents

miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for gain-of-function microRNA studies.

Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.

Highlights
  • Validated microRNA Mimic that targets Aldolase A in human, mouse, and rat
  • Same structure and design as experimental miRIDIAN microRNA Mimics
  • Allows optimization of assay conditions by monitoring mimic function on an endogenous gene target (Aldolase A) with a conserved miR-122 binding site (see Figure 1, Supporting Data)
  • Based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines, expressing low to moderate levels of miR-122
Applications
  • Link the activity of a microRNA mimic to post-transcriptional regulation of its putative gene target
  • Measure modulation of endogenous Aldolase A mRNA levels

Related Products

miRIDIAN Mimic Housekeeping Positive Control #1 (PPIB)

The miRIDIAN microRNA Mimic Housekeeping Positive Controls allow for the direct monitoring of housekeeping genes in a microRNA mimic experiment. Positive Control #1 targets PPIB (aka Cyclophilin B).

View Options
Mimic Housekeeping Positive Control #2 (GAPD)

The miRIDIAN microRNA Mimic Housekeeping Positive Controls allows for direct monitoring of housekeeping genes in a microRNA mimic experiment. Positive Control #2 targets GAPD so experimental conditions may be optimized with a validated mimic control.

View Options
miRIDIAN microRNA Mimic Red Transfection Control

The miRIDIAN microRNA Mimic Transfection Control is a Cy3-labeled microRNA mimic based on the C. elegans miRNA cel-miR-67 (miRIDIAN Mimic Negative Control #1) for monitoring delivery into human, mouse, and rat cells.

View Options
miRIDIAN microRNA Mimic Negative Control #1

Choose from two universal miRIDIAN Mimic Negative Controls for experiments with miRIDIAN microRNA Mimics. Negative control sequences based on C. elegans microRNAs have minimal sequence identity in human, mouse, and rat.

View Options