miRIDIAN microRNA Mimic Housekeeping Positive Control #2 (GAPD)
Validated miRIDAN microRNA Mimics designed to target GAPD in human, mouse and rat
The miRIDIAN microRNA Mimic Housekeeping Positive Controls allows for direct monitoring of housekeeping genes in a microRNA mimic experiment. Positive Control #2 targets GAPD so experimental conditions may be optimized with a validated mimic control.
miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for gain-of-function microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.
Highlights
- Same structure and design as experimental miRIDIAN microRNA Mimics
- Target the 3' UTR (untranslated region) of the standard housekeeping genes, PPIB, or GAPDH (see Figure 1, Supporting Data)
- Allows a clean, straightforward, cleavage-based readout of mimic function
Applications
- Delivery and function can be evaluated to determine transfection conditions in each cell line/type
- Monitor the function of a mimic molecule at the mRNA level using standard transcript quantification methods, such as RT-qPCR
