Custom Pooled Lentiviral Screening Libraries

Freedom with custom pooled library designs for your experimental needs. Your custom library can have:

  • Pools of lentiviral sgRNA, shRNA, or microRNA
  • Customizable promoter and reporter options, including inducible promoters
  • Complete workflow planning tools and protocols
  • Human, mouse, and rat options available

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Can't find a catalog screening library to meet your needs?

We can help you design your own custom lentiviral pools. Select from our range of algorithm-optimized reagents to develop the ideal screening resource for CRISPR-Cas9 knockout or RNAi knockdown screens.

Custom lentiviral pooled screening libraries offer an efficient method for screening large numbers of lentiviral sgRNAs, shRNAs, or microRNAs without the requirement of automation. All you need to do is select the product format, the number of genes, constructs per gene, and species.

Whether you need to perform a CRISPR knockout screen with lentiviral sgRNAs, a knockdown RNAi screen with shRNA, or a gene regulation screen with expressed microRNAs, a custom library can be built to your experimental requirements.

Ensure reproducibility and accurate hit identification

Library construction, pooling techniques, and high-throughput sequencing compatible screening workflows have all been experimentally validated. Pools are provided as concentrated lentiviral particles for transduction into dividing and non-dividing cells.

Experimentally validated protocols and calculation sheets are readily available to help you with every aspect of the experiment from determining relative titer in your specific cells to calculating exactly how much volume you will require to complete the experiment. Our Bioinformatic analysis protocol provides full instructions on using open source software to run the analysis from the NGS data.

To learn more about the critical parameters of successful pooled lentiviral screening, including the conditions necessary for maintaining a high fold-representation, please download the following publication: Ž. Strezoska, A. Licon, Optimized PCR Conditions and Increased shRNA Fold Representation Improve Reproducibility of Pooled shRNA Screens. PLoS One 7, e42341 (2012).

Specifications

Titer: Constitutive vectors, 5 x 108 TU/mL (± 20%); inducible vectors, 1 x 107 TU/mL (± 20%); all products are purified lentiviral particles with functional titers determined by qPCR. Pool size: minimum of 50 total constructs up to 12,000 constructs per pool Volume: Minimum of 100 uL up to 5 mL per pool; delivered in 25 µL aliquots