It’s hard to keep up with the rapidly expanding world of CRISPR, and it’s starting to feel like CRISPR screens are being published every week, taking the technique from the cutting edge to the mainstream. If you’d like to understand a bit more about CRISPR screens, here’s a number of fantastic publications that have really moved this technology forward.
A review of the main 5 tips to choose and use a cell line. We highlight the importance of checking for authenticity, and contamination.
Experimental results from a comparison of ON-TARGETplus siRNA and CRISPRi reagents reveals both technologies exhibit high specificity. This post also demonstrates the using the two in conjunction to produce a robust strategy for orthogonal hit validation.
Get a head start when planning your next RNA interference experiments with our most popular advice.
DharmaFECT™ transfection reagents provide multiple formulations for your specific application
Synthetic crRNA Positive Controls and DNA Mismatch Detection Primers
What factors should be considered in making the decision between using an siRNA individual or pool?
Choose modifications for siRNA and ssRNA