Accell 2.0 siRNA Controls

Accell 2.0 siRNA Control support optimization of siRNA delivery conditions and help monitor experimental variables using fluorescent or unlabeled positive and negative controls. Accell 2.0 siRNA Control Kits include species-specific controls designed to assess performance of Accell 2.0 siRNA Control Kits support optimization of siRNA delivery conditions and help monitor experimental variables using fluorescent or unlabeled positive and negative controls. Accell 2.0 siRNA Control Kits include species-specific controls designed to assess performance of Accell 2.0 siRNA in difficult-to-transfect cells. Convenient kits are available to support assay setup.

Experimental considerations

• Working concentration: Accell 2.0 siRNA is typically used at higher concentrations than conventional siRNA; ~1 µM is commonly recommended for efficient uptake and gene silencing.
• Serum conditions: Delivery may be reduced in high serum or BSA. Optimization using Accell 2.0 Delivery Media (serum-free) or ≤2.5% serum in standard media is recommended.
• Incubation timeline: Full-serum media can be added after ~48 hours. mRNA silencing is often observed by ~72 hours, with protein knockdown typically observed between 72-96 hours, depending on target turnover.