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Mimix™ MSI, FFPE Reference Standard

This MSI Reference standard contains 13 biomarkers relevant to determining the status of microsatellites in a patient sample, including markers in the National Cancer Institutes’ standardized Bethesda panel. Our reference standards are cell line-derived, for the closest representation of patient samples.

Microsatellites are repetitive sequences. Microsatellite Instability (MSI) relates to the malfunction of the DNA Mismatch Repair (MMR) genes that can introduce errors, in length or sequence, that result in novel repetitive sequences. MSI is most closely associated with colon cancers but shows prevalence in multiple other cancer types.

MSI is used in cancer diagnostics to aid the determination of an appropriate course of treatment for a patient. qPCR, fragment analysis or NGS workflows are used to determine MSI status in patient samples. This reference standard, manufactured under ISO:13485, can be used to validate assays which are designed to detect MSI status of any samples.

 

Technical Data

Format: FFPE

Fixation Method: 4% PFA (w/v)

Section Size: 15µm

Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section

Expected DNA Yield: >400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit

Markers covered:
BAT-25, BAT-26, BAT-40, CAT-25, NR-21, NR-22, NR-24, NR-27, ABI-16, ABI-17, ABI-19, ABI-20A, ABI-20B

Allelic frequencies: N/A

 

General Information

Storage: 4°C

Shipping: Ambient

Expiration: See all product shelf life information

 

Quality Control

MSI status: Fragment analysis

Quantification: Quantifluor

DNA Integrity: Agarose gel electrophoresis

Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.