Mimix OncoSpan controls are well-defined reference standards derived from a blend of cell lines, encompassing 386 variants across 152 essential cancer genes. These variants range from 1-100% allelic frequency (AF), with 51 variants present at ≤ 20% AF to aid in the limit of detection (LOD) determination of your assay. Each batch of Mimix OncoSpan controls include 25 variants confirmed by ddPCR, along with batch-specific whole exome sequencing (WES) at 500x coverage performed by Revvity Omics using the Agilent CREV3 kit on an Illumina platform. This helps to provide an accurate and reliable truth set for monitoring your workflow and assay's performance.

Upon ordering, you will receive a password to download the raw NGS data for your batch (including bam, bed, and vcf files). For added convenience, we also provide an easy-to-access Excel document listing the 386 high-confidence variants, available for download. 386 high confidence variants in an easy-to-access excel document.

Technical Product Information

Format: gDNA

Genes covered: ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDh2, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDh2, IDh4, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLh2, MLLT3, MMAB, MRE11, MSh4, MSH6, MTOR, NBN, NF1, NFE2L2, NOTCh1, NOTCh4, NOTCh6, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCh2, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395

Allelic frequencies: 1% - 92.5% for ddPCR-verified variants

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.7

Unit size:  1μg

Concentration:  50ng/μl

General information

Storage: 4°C

Shipping: Ambient

Expiration: See all product shelf life information

Intended Use:
The Mimix™ OncoSpan™ gDNA reference standard is a commutable control material comprising a mixture of genomic DNA (gDNA) derived from well characterized human cancer cell lines, containing more than 380 variants across 152 key cancer gene. It is intended for monitoring next-generation sequencing (NGS) and droplet digital polymerase chain reaction (ddPCR) assays designed to assist in the detection of somatic mutations in human gDNA samples for in vitro diagnostic use.

This control can be used to monitor NGS and ddPCR workflow, test performance, assay variation and helps identify increases in random or systematic errors. This product is for professional laboratory use only.

Quality control

Allelic frequency: Droplet Digital™ PCR or NGS

Quantification: Spectrophotometer

DNA Integrity: Agarose gel electrophoresis

Manufacturing Quality: ISO 13485:2016