Horizon’s Pan-Cancer 6-Fusion Panel (Negative Control) FFPE RNA Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of targeted NGS RNA-seq, endpoint reverse-transcription PCR (RT-PCR), RT-qPCR and RT droplet digital PCR (RT-ddPCR) assays aimed at detecting gene fusions.

This control was specifically designed to compliment Horizon's Pan-Cancer 6-Fusion Panel (HD834).

Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell-line verified not to contain TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions.

Technical Data

Format: FFPE

Genes Covered: Confirmed negative for TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET, SLC34A2-ROS1 

Fixation method: 10% Formalin

Section Size: 15 µm

Unit size: 1 x 15 µm FFPE section

Storage: 4˚C

Expiry: See all product shelf life information

Product Information

Verified Mutations:

Intended Use: For assay validation and routine monitoring of assay performance (for research use only)

Quality Control

Extractable RNA / Expected Yeild: ≥ 100 ng RNA per section using the Maxwell RSC® RNA FFPE Purification Kit (Promega)

RNA Quantification: Qubit® RNA HS Assay (Thermo Fisher Scientific)

Fusion Biomarker Expression: Absence of TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA transcripts confirmed by fusion specific RT-ddPCR using the QX200 ddPCR System (Bio-Rad)

RNA Integrity: Expected DV200 ≥65% assessed using TapeStation High Sensitivity RNA ScreenTape Assay (Agilent) 

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.