Mimix™ Pan-Cancer 6-Fusion Panel, FFPE Reference Standard

Defined FFPE control material containing 6 clinically-relevant, cell-based kinase fusion RNA biomarkers 

Product name change
As part of the continuous evolution of our portfolio, we have changed the name of our range to Mimix™ reference standards to better reflect the patient-like performance they provide.

While our product range has a new name, everything you rely on remains the same:

  • Identical formulation and performance
  • Same part numbers and SKUs
  • Existing protocols and procedures
  • Same technical specifications
  • Quality and reliability you trust

No action is required from you. Future orders, product labels, documentation, and communications will reference the new product name.

Horizon's Pan-Cancer 6-Fusion Panel FFPE RNA Reference Standard is a highly characterized, clinically-relevant, cell line-derived quality control material used to assess the performance of targeted NGS RNA-Seq, endpoint reverse-transcription PCR (RT-PCR), RT-qPCR and RT droplet digital (RT-ddPCR) assays aimed at detecting gene fusions. 

Each 15µm section contains a multiplexed blend of formalin-fixed paraffin-embedded (FFPE) cell lines verified to contain 6 kinase fusion RNA biomarkers in the complexity of the cell transcriptome. 

 

Product Highlights

  • Contains popular NTRK gene fusions as well as ALK, RET and ROS 
  • 100% cell-line derived reference standards that mimics patient samples
  • Consistent product specification from batch to batch
  • Suitable for workflow NGS assay performance
  • Quality controlled with digital PCR for accurate quantitative target mutations
  • Manufactured under ISO 9001 certification

 

Technical Data

Format: FFPE

Genes Covered: TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET, SLC34A2-ROS1 

Fixation method: 10% Formalin

Section Size: 15 µm

Unit size: 1 x 15 µm FFPE section

Storage: 4˚C

Expiry: See all product shelf life information

 

Product Information

Verified Mutations:

5' Partner 5' Partner Chromosome 3' Partner 3' Partner Chromosome RNA Expression Level

TPM3 (Exon 7)

1

NTRK1 (Exon 10)

1

 ≥100 Copies/ng

QKI (Exon 6)

6

NTRK2 (Exon 16)

9

 ≥100 Copies/ng

ETV6 (Exon 5)

12

 NTRK3 (Exon 15)

15

 ≥4 Copies/ng

EML4 (Exon 13)

2

ALK (Exon 20)

2

 ≥40 Copies/ng

CCDC6 (Exon 1)

10

RET (Exon 12)

10

 ≥4 Copies/ng
SLC34A2 (Exon 4)

4

ROS1 (Exon 32)

6

 ≥40 Copies/ng

Quality Control

Extractable RNA / Expected Yield: ≥ 100 ng RNA per section using the Maxwell RSC® RNA FFPE Purification Kit (Promega)

RNA Quantification: Qubit® RNA HS Assay (Thermo Fisher Scientific)

Fusion Biomarker Expression: Expression of TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA transcripts confirmed by fusion specific RT-ddPCR using the QX200 ddPCR System (Bio-Rad)

RNA Integrity: Expected DV200 ≥65% assessed using TapeStation High Sensitivity RNA ScreenTape Assay (Agilent) 

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.

Related Products

Mimix™ Pan-Cancer 6-Fusion Panel Negative Control, FFPE Reference Standard

Defined cell-line derived FFPE control material, verified as negative for TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions

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