Our website has a fresh new "look"
We've updated our site with modern design and easier navigation, but you'll still find the same quality Dharmacon™ reagents and Mimix™ reference standards that you love.
Mimix™ Pan-Cancer 6-Fusion Panel, FFPE Reference Standard
Horizon's Pan-Cancer 6-Fusion Panel FFPE RNA Reference Standard is a highly characterized, clinically-relevant, cell line-derived quality control material used to assess the performance of targeted NGS RNA-Seq, endpoint reverse-transcription PCR (RT-PCR), RT-qPCR and RT droplet digital (RT-ddPCR) assays aimed at detecting gene fusions.
Each 15µm section contains a multiplexed blend of formalin-fixed paraffin-embedded (FFPE) cell lines verified to contain 6 kinase fusion RNA biomarkers in the complexity of the cell transcriptome.
Product Highlights
- Contains popular NTRK gene fusions as well as ALK, RET and ROS
- 100% cell-line derived reference standards that mimics patient samples
- Consistent product specification from batch to batch
- Suitable for workflow NGS assay performance
- Quality controlled with digital PCR for accurate quantitative target mutations
- Manufactured under ISO 9001 certification
Technical Data
Format: FFPE
Genes Covered: TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET, SLC34A2-ROS1
Fixation method: 10% Formalin
Section Size: 15 µm
Unit size: 1 x 15 µm FFPE section
Storage: 4˚C
Expiry: See all product shelf life information
Product Information
Verified Mutations:
| 5' Partner | 5' Partner Chromosome | 3' Partner | 3' Partner Chromosome | RNA Expression Level |
|---|---|---|---|---|
|
TPM3 (Exon 7) |
1 |
NTRK1 (Exon 10) |
1 |
≥100 Copies/ng |
|
QKI (Exon 6) |
6 |
NTRK2 (Exon 16) |
9 |
≥100 Copies/ng |
|
ETV6 (Exon 5) |
12 |
NTRK3 (Exon 15) |
15 |
≥4 Copies/ng |
|
EML4 (Exon 13) |
2 |
ALK (Exon 20) |
2 |
≥40 Copies/ng |
|
CCDC6 (Exon 1) |
10 |
RET (Exon 12) |
10 |
≥4 Copies/ng |
| SLC34A2 (Exon 4) |
4 |
ROS1 (Exon 32) |
6 |
≥40 Copies/ng |
Quality Control
Extractable RNA / Expected Yield: ≥ 100 ng RNA per section using the Maxwell RSC® RNA FFPE Purification Kit (Promega)
RNA Quantification: Qubit® RNA HS Assay (Thermo Fisher Scientific)
Fusion Biomarker Expression: Expression of TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA transcripts confirmed by fusion specific RT-ddPCR using the QX200 ddPCR System (Bio-Rad)
RNA Integrity: Expected DV200 ≥65% assessed using TapeStation High Sensitivity RNA ScreenTape Assay (Agilent)
Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Related Products
Defined cell-line derived FFPE control material, verified as negative for TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions