The GIPZ microRNA-adapted shRNA collection was developed in collaboration with Gregory Hannon and Steve Elledge at Cold Spring Harbor Laboratories (CSHL). GIPZ shRNA designs are based on native miR-30 primary transcript to enable processing by the endogenous RNAi pathway and result in specific gene silencing with minimized cellular toxicity.

Highlights

• microRNA-adapted shRNA for specific knockdown
• TurboGFP marks cells expressing shRNA
• Genome-wide human and mouse representation
• Lentiviral delivery extends RNAi to primary and non-dividing cells
• Generation and maintenance of stable cell lines facilitated by puromycin selection marker

Pre-designed human and mouse GIPZ shRNA designs are available as bacterial glycerol stocks or high-titer lentiviral particles. Search for your gene(s) of interest to find

• Individual shRNA constructs, supplied as E. coli glycerol stocks or high-titer lentiviral particles (1x10⁸ TU/mL, ± 20%, functional titer obtained by flow cytometric analysis of transduced GFP-positive HEK293T cells)
• shRNA Starter Kits which include positive and negative controls, plus delivery reagent
• Target Gene Sets comprised of 3 to 6 GIPZ shRNA constructs

*One out of three GIPZ Lentiviral shRNA is guaranteed to produce gene knockdown by ≥ 70% at the mRNA level when used in shRNA Starter Kit. Knockdown should be compared to non-silencing control and experimental workflow must be controlled using the provided GIPZ GAPDH positive control.

GIPZ Lentiviral shRNA Starter Kits

• Your choice of GIPZ lentiviral shRNA constructs targeting human or mouse genes
• Positive and negative GIPZ lentiviral shRNA controls
• Kits for delivery of GIPZ shRNA by transfection or transduction
• Lentiviral particle starter kits provided as 2 x 25 µL aliquots of each gene-targeting construct, 1 x 25 µL aliquots of each control
• Guaranteed knockdown when at least 3 GIPZ shRNAs are tested following protocols and using the included GIPZ shRNA positive and negative controls

The GIPZ Transduction Starter Kit includes:

• Three GIPZ shRNA as E. coli glycerol stock cultures
• Trans-Lentiviral packaging mix sufficient for 10 packaging events
• Calcium phosphate transfection reagent
• Positive and negative GIPZ lentiviral shRNA controls‡
• Guaranteed knockdown† when at least 3 GIPZ shRNAs are tested following protocols and using the included GIPZ shRNA positive and negative controls

The GIPZ Lentiviral shRNA Transfection Starter Kit with DharmaFECT kb includes:

• Three GIPZ shRNA as E. coli glycerol stock cultures
• Positive and negative GIPZ lentiviral shRNA controls
• DharmaFECT transfection reagent for delivery of shRNA plasmid
• Guaranteed knockdown† when at least 3 GIPZ shRNAs are tested following protocols and using the included GIPZ shRNA positive and negative controls

Transduction-ready high-titer lentiviral particle format reduces time, labor, and costs involved in prepping DNA, packaging and purifying lentiviral particles for your RNAi experiments. Concentrated, high-titer lentiviral particles allow multiple experiments in a variety of cell types requiring variable multiplicity of infection (MOI). Lentiviral particles are provided at titers of 1x10⁸ TU/mL, ± 20%, functional titer obtained by flow cytometric analysis of transduced GFP-positive HEK293T cells).

Please note that due to the inherent complexity of high-titer lentiviral particle production, we estimate a 6 to 8 week turnaround time.

† When you purchase a minimum of three shRNA to the same target, at least one of the shRNA constructs will reduce target mRNA levels by 70% or more when used with Starter Kit protocols and normalized for delivery efficiency. Delivery conditions should be confirmed using appropriate positive controls provided in the kit and the percent-knockdown should be compared to cells using the negative control (non-targeting) shRNA.

Note that the GIPZ Lentiviral shRNA and TRIPZ Inducible Lentiviral shRNA are not compatible with third generation packaging systems, such as ViraPower from Invitrogen. We recommend the Trans-Lentiviral Packaging System to general lentiviral particles for transduction of GIPZ and TRIPZ shRNA.