Mimix OncoSpan controls are a well-characterized, cell line-derived pan-cancer reference standard, featuring over 380 variants across 152 key cancer genes. These variants range from 0.9% -100% allelic frequency (AF), with 60 variants present at ≤ 20% AF to aid in the limit of detection (LOD) determination of your assay. Each batch of Mimix OncoSpan controls include 25 variants confirmed by ddPCR, along with batch-specific whole exome sequencing (WES) at 500x coverage performed by Revvity Omics using the Agilent CREV3 kit on an Illumina or Aviti platform. This helps to provide an accurate and reliable truth set for monitoring your workflow and assay's performance.

Upon ordering, you will receive a password to download the raw NGS data for your batch (including bam, bed, and vcf files). For added convenience, we also provide an easy-to-access Excel document listing the 386 high-confidence variants, available for download: 386 high confidence variants in an easy-to-access excel document.

Technical Product Information

Format: FFPE

Fixation method: 4% PFA (w/v)

Section size: 15µm

Cell density 3 x 10⁸ cells per block. Approx. 3.5 x 10⁵ cells per section

Expected DNA yield: > 400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit

Genes covered: ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDh2, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDh2, IDh4, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLh2, MLLT3, MMAB, MRE11, MSh4, MSH6, MTOR, NBN, NF1, NFE2L2, NOTCh1, NOTCh4, NOTCh6, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCh2, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395

Allelic frequencies: 0.9% - 92.5% for ddPCR-verified variants

General information

Storage: 4°C

Shipping: Ambient

Expiration: See all product shelf life information

Intended Use Statement:
The Mimix™ OncoSpan™ FFPE reference standard is a commutable control material comprising a formalin-fixed paraffin-embedded (FFPE) curl derived from well characterized human cancer cell lines, containing more than 380 variants across 152 key cancer genes. It is intended for monitoring next-generation sequencing (NGS) or droplet digital polymerase chain reaction (ddPCR) assays designed to assist in the detection of somatic mutations in genomic DNA (gDNA) from human samples for in vitro diagnostic use.

The gDNA obtained from the Mimix OncoSpan FFPE Reference Standard can be used to monitor NGS or ddPCR workflow, test performance, assay variation and helps identify increases in random or systematic errors. This product is for professional laboratory use only.

Intended Use: For In Vitro Diagnostic Use Only.

Quality control

Allelic frequency: Droplet Digital™ PCR or NGS

Quantification: Quantifluor

DNA Integrity: Agarose gel electrophoresis

Manufacturing Quality: ISO13485

Mimix OncoSpan publications

Read these research articles citing the use of Mimix OncoSpan reference standards.

• Validation of a pan-cancer targeted next generation sequencing panel in New Zealand | New Zealand Journal of Medical Laboratory Science
• Enabling variant calling in challenging FFPE samples by coupling a novel library preparation chemistry with exome sequencing | Journal of Clinical Oncology
• Moving towards a local testing solution for undetermined thyroid fine-needle aspirates: validation of a novel custom DNA-based NGS panel | Journal of Clinical Pathology
• Performance Analysis of Three Bioinformatic Variant Callers Using a Somatic Reference Standard | Children's Mercy Kansas City