Between choosing the right reagents and detecting changes at the genomic level, many factors need to be considered to create a well-designed CRISPR-Cas9 experiment.
An overview of design rules, benefits, and limitations of T7EI detection for CRISPR experiments
Check out the BioIT Beta Tools page to test tools created by our BioIT team!
CRISPR-Cas9 can be used to knockout specific microRNAs in cell lines
Tips and tricks for RNAi success - make sure to include necessary controls in your transfections!
A gene editing revolution is underway in plant systems
Loss-of-function screening on hundreds to tens of thousands of genes at once
When you need to knockout a gene next week, not next month
DharmaFECT kb: A DNA transfection reagent with a large dynamic range and low cytotoxicity!
What you need to know about variables that affect efficiency of HDR knock-in using CRISPR-Cas9