A study where we have employed orthogonal target validation strategies with 3 orthogonal LOF technologies (CRISPRko, CRISPRi, and siRNA) with genes involved in maintenance of the mesenchymal-to-epithelial transition (MET).
The presence of off-targets effects can greatly complicate the interpretation of experimental data. Dharmacon™ ON-TARGETplus™ siRNAs and CRISPRmod CRISPRi system are designed to knock down the target gene with minimal off-target effects and are powerful tools for orthogonal validation strategies resulting in highly specific readouts.
Accell siRNA has been widely literature-cited for gene silencing in primary immune cells, which have historically been difficult to transfect. Learn more here.
Learn how ON-TARGETplus siRNA from Dharmacon ensures target specificity while avoiding off-target effects to give you confidence in your RNAi studies.
Want to optimize RNAi experiments in difficult-to-transfect cell lines? Read our workflow recommendations for optimal delivery and functional silencing with Accell siRNA.
Tips and tricks for RNAi success - make sure to include necessary controls in your transfections!
The only self-delivering siRNA for difficult-to-transfect cells carries a functional guarantee.