3D culturing is a valuable and increasingly popular cell culture technique. This model recapitulates some physiological characteristics of tissues and tumors driven by cell-cell and cell-matrix contacts, circumventing the need for in vivo experiments in some cases.
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A study where we have employed orthogonal target validation strategies with 3 orthogonal LOF technologies (CRISPRko, CRISPRi, and siRNA) with genes involved in maintenance of the mesenchymal-to-epithelial transition (MET).
The presence of off-targets effects can greatly complicate the interpretation of experimental data. Dharmacon™ ON-TARGETplus™ siRNAs and CRISPRmod CRISPRi system are designed to knock down the target gene with minimal off-target effects and are powerful tools for orthogonal validation strategies resulting in highly specific readouts.
CRISPR gene editing and siRNA gene silencing require the efficient delivery of the necessary reagents. This is easily achieved by optimizing delivery conditions. In this blog post we discuss techniques for optimization of a reverse transfection conditions and how to set up a 96 well plate to test 30 transfection conditions.
Accell siRNA has been widely literature-cited for gene silencing in primary immune cells, which have historically been difficult to transfect. Learn more here.
Learn how ON-TARGETplus siRNA from Dharmacon ensures target specificity while avoiding off-target effects to give you confidence in your RNAi studies.
Want to optimize RNAi experiments in difficult-to-transfect cell lines? Read our workflow recommendations for optimal delivery and functional silencing with Accell siRNA.
Learn how to use our Dharmacon™ siDESIGN center for isoform or gene variant-specific siRNAs
Interested in prime editing? Learn about this new technique and how Horizon's customizable long oligo synthesis can help get you started.
Are you new to RNAi experiments and need some guidance on troubleshooting?