What do I need to consider before initiating an in vivo study?

Due to the complexity of in vivo RNAi studies, the experimental design includes many considerations: animal model, delivery method, biodistribution, residence time in the body before clearance, dosage, frequency of dosing regimen, controls, and approval of your protocols by any institutional review board. For synthetic siRNAs, one must also consider amount needed (size of recipient, number in study, delivery method), modifications (dyes; stabilizing modifications for RNAase rich environment), and special processing. We recommend starting with an in vitro pilot study before continuing with a small animal cohort. Although, we don´t perform in house experiments, here are some references/ documents, which might be useful: In vivo RNAi: Biodistribution, delivery, and applications:
https://horizondiscovery.com/-/media/Files/Horizon/resources/Application-notes/in-vivo-technote.pdf

Dharmacon siRNA in vivo references: https://horizondiscovery.com/-/media/Files/Horizon/resources/Reading-lists/sirna-in-vivo-ref-reading.pdf

Option in vivo and in vivo HPLC Custom siRNA Synthesis”: https://horizondiscovery.com/-/media/Files/Horizon/resources/Product-inserts/option-invivo-hplc-prodinsert.pdf