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Custom siRNA synthesis
siRNA customization has never been easier
No pre-designed product to fit your needs? Use our online design tools and extensive synthesis options to create a custom siRNA specific for your application. Numerous combinations of modifications, sizes, and purification options are available for convenient online ordering. Try the tools now or watch a short tutorial video on how-to-use the siRNA custom tool to quickly generate an online quote for your custom siRNA.
Order custom siRNA
Order both modified or unmodified siRNAs. Patented modification patterns (ON-TARGET, ON-TARGETplus, siSTABLE, or Accell) and additional custom modifications are available.
Custom SMARTpool design & synthesis
To complement our pre-designed siRNA collections and support a broad range of RNAi experiments, our siRNA design experts can provide custom SMARTpool reagents targeting genes outside of our genome wide offerings.
siDESIGN Center
Design siRNAs targeting genes in non-standard species, particular splice variants or homologous regions across gene families or species.
Preclinical scale and OEM RNA & DNA synthesis
Preclinical scale/OEM service for synthesis of RNA and DNA at larger quantities.
Alternatively, if you would prefer a custom generated quote fill out the custom synthesis quote form.
siRNA Yield Table
For unmodified siRNA, the following approximate yields can be expected:
Standard (A4) | HPLC purified | in vivo | in vivo HPLC | |||||
---|---|---|---|---|---|---|---|---|
nmol | mg | nmol | mg | nmol | mg | nmol | mg | |
0.025 µmol scale | 20 | 0.25 | - | - | - | - | - | - |
0.05 µmol scale | 40 | 0.5 | 20 | 0.25 | 25 | .3 | - | - |
0.2 µmol scale | 150 | 2 | 80 | 1 | 100 | 1.3 | 50 | 0.65 |
0.4 µmol scale | 300 | 4 | 160 | 2 | 200 | 2.6 | 100 | 1.3 |
1.0 µmol scale | 750 | 10 | 320 | 4 | 500 | 6.6 | 250 | 3.3 |
2.0 µmol scale | 1500 | 20 | 750 | 10 | 1000 | 13 | 500 | 6.6 |
5.0 µmol scale | 3750 | 50 | 1875 | 25 | 2500 | 33 | 1250 | 16 |
10.0 µmol scale | 7500 | 100 | 3750 | 50 | 5000 | 66 | 2500 | 33 |
Expert siRNA design assistance is available
Our capabilities include a range of alternative siRNA designs to empower your RNAi research
If you are working in human, mouse or rat models, we may already have what you need as a pre-designed product: Search for your gene in the Search field found in the upper right corner.
Take advantage of our internal design experts and SMARTpool technology! Order a functionally guaranteed Custom SMARTpool for worry-free customization.
Alternative siRNA designs that we support include:
- Blunt-ended
- Asymmetrical
- Longer duplexes (>23 nt)
- Mismatched strands
- Alternative bases or linkages
If you can't find what you need or you require additional specifications not available through online order, please request a quote.
For further assistance, please contact Technical Support.
Modified for specificity, stability or self-delivery, we have the siRNA solution.
Choose one of our specialized chemical modification patterns to enhance your custom siRNA.
All siRNA modifications, proprietary and other, are available through custom siRNA synthesis
ON-TARGET | ON-TARGETplus | Accell | siSTABLE | |
---|---|---|---|---|
Inhibits sense (passenger) strand uptake by RISC | ✔ | ✔ | ✔ | ✔ |
Antisense strand seed region modified for greater specificity to target | ✔ | |||
Resistant to endo-and exonuclease degradation | ✔ | ✔ | ||
Delivery into cells without transfection reagent | ✔ | |||
Also available as Pre-designed siRNA | ✔ | ✔ |
ON-TARGET modification ensures antisense strand bias

The ON-TARGET modification promotes correct strand uptake by blocking the sense (passenger) strand from being taken up by the RISC process. This is a useful method for ensuring antisense (guide) strand processing. All proprietary siRNA modifications (ON-TARGETplus, Accell, siSTABLE) incorporate this sense-strand modification to promote antisense (guide) strand-facilitated silencing.
ON-TARGETplus siRNA dual-strand modification pattern for reduction of off-targets

A 2006 publication demonstrates that off-target effects are primarily driven by antisense strand seed activity.1 Therefore, sense strand inactivation alone does not decrease the total number of off-target genes. ON-TARGETplus modifications account for both strands:
- Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
- Antisense strand seed region is modified to minimize seed-related off-targeting
The ON-TARGETplus modification pattern dramatically reduces off-targets. Off-target effects induced by the indicated siRNAs were quantified using microarray analysis. For each target, three different siRNAs were used: unmodified, sense strand-inactivated, and ON TARGETplus-modified. Data shown represents genes down-regulated by two-fold or more. HEK293 cells were transfected with 100 nM siRNA using 0.2 µL of DharmaFECT 1. Data was analyzed at 24 hours.
1 Jackson, A.L. et al. "Position-specific Chemical Modification Increases Specificity of siRNA-mediated Gene Silencing." RNA 12.7 (2006) 1197-1205.
Accell delivery and gene silencing in cardiomyocytes without a transfection reagent

Neonatal rat ventricular myocytes were incubated with 1 µM Accell Green (A; Cat# D-001950-01) or Red (B; Cat# D-001960-01) Non-targeting siRNA for 72 hours in Accell delivery media (Cat# B-005000). Nuclei were stained with DAPI (blue). Labeled control uptake showed diffuse cytoplasmic localization in nearly all cells.
The bar graph indicates the level of gene silencing achieved with Accell GAPD Control siRNA (Cat# D-001930-03) and Pool (Cat# D-001930-30) control reagents when used with neonatal rat ventricular myocyte (NRVM) media or Accell delivery media.
Myocytes were prepared as described in Maass AH & Buvoli M. Cardiomyocyte preparation, culture, and gene transfer. Methods Mol Biol. 2007;366: 321-30. mRNA expression was determined by QuantiGene branched DNA assay (Panomics).
siSTABLE-modified siRNAs resist degradation by nucleases

Conventional siRNA is degraded within minutes in serum-containing environments, making in vivo use of siRNA problematic. This graph presents evidence that the siSTABLE modification pattern dramatically extends the half-life of siRNA in the presence of 100% human serum as compared to Stealth RNAi (Invitrogen).
Accell siRNA also includes these stability-enhancing modifications, as well as providing delivery to difficult-to-transfect cells without a transfection reagent.
Fluorophore | λ max abs (nm) | λ max em (nm) | Comparable to | 5' or 3' | |
---|---|---|---|---|---|
Fluorescein / 6-FAM
5'-Fluorescein![]() Description: Fluorescein is often used in fluorescence experiments to demonstrate the kinetics of folding or substrate binding. Fluorescein is also used as a donor to track optimal changes related to folding or substrate binding to intermolecular interactions.
Reference: Science 266: 785-789 (1994), EMBO J. 17: 2378-2391 (1998) |
494 | 520 | - | both* | |
TAMRA
5'-TAMRA-hexyl linker![]() Description: TAMRA is a strongly absorbing dye with a wide variety of applications. This modification is coupled from the 5'- or 3'-end of an oligonucleotide to either the 5th or 6th position of the dye.
References: Nucl. Acids. Res. 24: 4535-4542 (1996), Biochem. 39: 14487-14484 (2000) |
565 | 580 | - | both* | |
Cy3
Modification Name: 5’-Cy3![]() Modification Code: Cy3 Unit Molecular Weight: 507.59 g/mol Cy3 Extinction Coefficient: 136,000 Excitation/Emission Max: 547 nm/563 nm Unit Structure: Modification Name: 3’-Cy3![]() Modification Code: Cy3-3’ Unit Molecular Weight: 800.85 g/mol Cy3 Extinction Coefficient: 136,000 Excitation/Emission Max: 547 nm/563 nm Unit Structure: |
547 | 563 | - | both* | |
Cy5
Modification Name: 5’-Cy5![]() Modification Code: Cy5 Unit Molecular Weight: 533.63 g/mol Cy5 Extinction Coefficient: 250,000 Excitation/Emission Max: 646 nm/662 nm Unit Structure: Modification Name: 3’-Cy5![]() Modification Code: Cy5-3’ Unit Molecular Weight: 826.88 g/mol Cy5 Extinction Coefficient: 250,000 Excitation/Emission Max: 646 nm/662 nm Unit Structure: |
646 | 662 | - | both* | |
Cy5.5
Modification Name: 5’-Cy5.5![]() Modification Code: Cy5^5 Unit Molecular Weight: 633.75 g/mol Cy5.5 Extinction Coefficient: 209,000 Excitation/Emission Max: 688 nm/707 nm Unit Structure: Modification Name: 3’-Cy5.5![]() Modification Code: Cy5^5-3’ Unit Molecular Weight: 927.00 g/mol Cy5.5 Extinction Coefficient: 209,000 Excitation/Emission Max: 688 nm/707 nm Unit Structure: |
688 | 707 | - | both* |
Some of the dye options listed in the above tables will produce higher oligo yields than others. For assistance in choosing the most appropriate dye label for your application, please contact Technical Support. You can also request a quote online if you already know that an alternative dye will be required for your experiments.
Alexa Fluor® is a registered trademark of Invitrogen Corporation.
In addition to fluorescent dyes, we offer a broad portfolio of other chemical modifications that can also be applied to both siRNA and single-strand RNA.
For information on our specialized modification patterns for reducing off-targets, self-delivery and nuclease resistance, view the Proprietary siRNA tab.

siRNA can be modified at either end, on either strand. Multiple modifications are also available but availability may be dependent upon sequence or other factors. Please contact Technical Support for more information
We offer a wide array of oligonucleotide modifications for your specific custom synthesis applications. Scroll through RNA type and position below for online ordering availability.
NOTE: Not all modifications are available through online ordering tool. For assistance please contact Technical Support.
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Showing modifications available for:
- siRNA
- ssRNA
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Standard RNA Bases | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
---|---|---|---|---|---|---|
(A,C,G,U)
Standard RNA Bases (A, C, G, U)
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
A,C,G,U | $6.50 | $8.20 | $13.70 | $18.00 | $27.60 |
2'-Omethyl RNA Bases | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
2'-OMe RNA Bases (A, C, G, U)
2'-OMe RNA Bases (A, C, G, U)
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
mA,mC,mG,mU | $6.50 | $8.20 | $13.70 | $18.00 | $27.60 |
Standard DNA Bases | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
2'-Deoxy Bases (A, C, G, T)
2'-Deoxy Bases (A, C, G, T)
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
dA,dC, dG, dT | $6.50 | $8.20 | $13.70 | $18.00 | $27.60 |
Base Modifications | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
1-Methyl-guanosine
1-Methyl-guanosine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight:
References: |
m1G | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
2,6-Diaminopurine
2,6-Diaminopurine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
DAP | $90.00 | $95.00 | $105.00 | $135.00 | $230.00 |
2-Methyl-adenosine
2-Methyl-adenosine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight:
References: |
m2A | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
2-Aminopurine
2-Aminopurine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2AP | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
4-Thio-uridine
4-Thio-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
4-S-U | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
5-Bromo-Uridine
5-Bromo-Uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
U[5Br] | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Fluoro-cytidine
5-Fluoro-cytidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
C[5F] | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Fluoro-uridine
5-Fluoro-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
U[5F] | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Iodo-uridine
5-Iodo-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
U[5I] | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Methyl-cytidine
5-Methyl-cytidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
5-M-C | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Methyl-Deoxycytidine
5-Methyl-Deoxycytidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: References: Freier, S. M. and Altmann, K.-H. The ups and downs of nucleic acid duplex stability: structure-stability studies on chemically modified DNA:RNA duplexes. Nucleic Acid Res. 25, 4429-4443 (1997). |
5-M-dC | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Methyl-uridine
5-Methyl-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
rT | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
Inosine
Inosine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
I | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
N3-Methyl-uridine
N3-Methyl-uridine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight:
References: |
3-M-U | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
N6,N6-Dimethyl-adenosine
N6,N6-Dimethyl-adenosine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
DMA | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
N6-Methyl-adenosine
N6-Methyl-adenosine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
m6A | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
Pseudouridine
Pseudouridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
~U | $90.00 | $95.00 | $105.00 | $135.00 | $230.00 |
Purine ribonucleoside
Purine ribonucleoside
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
Pu | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
Pyrrolo-cytidine
Pyrrolo-cytidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
pC | $90.00 | $95.00 | $105.00 | $135.00 | $230.00 |
Ribavirin
Ribavirin
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
RBV | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
Backbone modifications | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
Phosphorothioate
Phosphorothioate
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
* | $8.00 | $13.00 | $15.00 | $20.00 | $29.00 |
Phosphorylation | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
5'-Phosphate
5'-Phosphate
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
5'-P | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
2'-Modifications | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
2'-Amino-butyryl-pyrene-uridine
2'-Amino-butyryl-pyrene-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Pyrene Extinction Coefficient:
Excitation/Emission Max:
References: |
2'-P-U | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
2'-Amino-cytidine
2'-Amino-cytidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2'-N-C | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
2'-Amino-uridine
2'-Amino-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2'-N-U | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
2'-Deoxy-uridine
2'-Deoxy-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
dU | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
2'-Fluoro-adenosine
2'-Fluoro-adenosine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2'-F-A | $58.00 | $60.00 | $85.00 | $87.00 | $160.00 |
2'-Fluoro-cytidine
2'-Fluoro-cytidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2'-F-C | $40.00 | $43.00 | $57.00 | $65.00 | $100.00 |
2'-Fluoro-guanosine
2'-Fluoro-guanosine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2'-F-G | $58.00 | $60.00 | $85.00 | $87.00 | $160.00 |
2'-Fluoro-uridine
2'-Fluoro-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
2'-F-U | $40.00 | $43.00 | $57.00 | $65.00 | $100.00 |
2'-OMe-inosine
2'-OMe-inosine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
mI | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
Amino Modifiers | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
3'-Amino modifier C12
3'-Amino modifier C12
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
N12-3' | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
3'-Amino modifier C6
3'-Amino modifier C6
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
N6-3' | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
5'-Amino modifier C12
5'-Amino modifier C12
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
N12 | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
5'-Amino modifier C3
5'-Amino modifier C3
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
N3 | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5'-Amino modifier C5
5'-Amino modifier C5
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
N5 | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5'-Amino modifier C6
5'-Amino modifier C6
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
N6 | $73.00 | $80.00 | $90.00 | $115.00 | $200.00 |
5-Aminohexylacrylamino-uridine
5-Aminohexylacrylamino-uridine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
5-LC-N-U | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
Thiol Modifiers | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
3'-Disulfide thiol-modifier
3'-Disulfide thiol-modifier
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
S-S-3' | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
5'-Disulfide thiol-modifier
5'-Disulfide thiol-modifier
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
S-S | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
Spacer Modifiers | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
C18 Spacer
C18 Spacer
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
18S | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
C3 Spacer
C3 Spacer
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
C3 | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
C9 Spacer
C9 Spacer
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
9S | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
dSpacer
dSpacer
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: References: Vesnaver, G. et al. Influence of abasic and anucleosidic sites on the stability, conformation, and melting behavior of a DNA duplex: correlations of thermodynamic and structure data. Proc. Natl. Acad. Sci. USA 86, 3614-3618 (1989). Taniho, K. et al. Synthesis and biological properties of chemically modified siRNAs bearing 1-deoxy-D-ribofuranose in their 3'-overhang region. Bioorg. Med. Chem. Lett. 22, 2518-2521 (2012). |
dab | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
rSpacer
rSpacer
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: References: Vesnaver, G. et al. Influence of abasic and anucleosidic sites on the stability, conformation, and melting behavior of a DNA duplex: correlations of thermodynamic and structure data. Proc. Natl. Acad. Sci. USA 86, 3614-3618 (1989). Taniho, K. et al. Synthesis and biological properties of chemically modified siRNAs bearing 1-deoxy-D-ribofuranose in their 3'-overhang region. Bioorg. Med. Chem. Lett. 22, 2518-2521 (2012). |
rab | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
Chain Terminators | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
3' Inverted abasic
3' Inverted abasic
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
b | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
3' Inverted deoxy-thymidine
3' Inverted deoxy-thymidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
idT-3' | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
3'-Terminal 3'-Deoxy-Guanosine
3'-Terminal 3'-Deoxy-Guanosine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
3'dG | $55.00 | $80.00 | $90.00 | $120.00 | $180.00 |
3'-Terminal dideoxy-cytidine
3'-Terminal dideoxy-cytidine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
ddC | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
5' Inverted deoxy-thymidine
5' Inverted deoxy-thymidine
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
idT | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
5' Terminal 5'-deoxy-ribo-adenosine
5' Terminal 5'-deoxy-ribo-adenosine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
5'dA | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
5' Terminal 5'-deoxy-ribo-cytidine
5' Terminal 5'-deoxy-ribo-cytidine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
5'dC | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
5' Terminal 5'-deoxy-ribo-guanosine
5' Terminal 5'-deoxy-ribo-guanosine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
5'dG | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
5' Terminal 5'-deoxy-ribo-uridine
5' Terminal 5'-deoxy-ribo-uridine
Modification Code: Unit Structure: ![]()
Unit Molecular Weight: |
5'dU | $30.00 | $32.00 | $42.00 | $58.00 | $100.00 |
Labeling | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
3-Biotin
3-Biotin
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
3'-Bi | $75.00 | $95.00 | $105.00 | $130.00 | $190.00 |
3'-Cholesterol
3'-Cholesterol
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
3'-Chl | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
3' TEG-Cholesterol
3' TEG-Cholesterol
Modification Code:
Description:
Unit Structure: ![]()
Chemical Formula:
Unit Molecular Weight:
References:
|
TEG-Chl-3' | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
3'-Cy3
3'-Cy3
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Cy3 Extinction Coefficient:
Excitation/Emission Max:
References: |
Cy3-3' | $85.00 | $105.00 | $125.00 | $145.00 | $270.00 |
3'-Cy5
3'-Cy5
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Cy5 Extinction Coefficient:
Excitation/Emission Max:
References: |
Cy5-3' | $85.00 | $105.00 | $125.00 | $145.00 | $270.00 |
3'-Cy5.5
3'-Cy5.5
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Cy5.5 Extinction Coefficient:
Excitation/Emission Max:
References: |
Cy5^5-3' | $85.00 | $105.00 | $125.00 | $145.00 | $270.00 |
3’ Black Hole Quencher 1
3’ Black Hole Quencher 1
|
BHQ-1-3’ | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
3’ Black Hole Quencher 2
3’ Black Hole Quencher 2
|
BHQ-2-3’ | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
3'-Fluorescein
3'-Fluorescein
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Extinction Coefficient:
Excitation/Emission Max: |
3'-Fl | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
3'-Biotin LC
3'-Biotin LC
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
3'-LCBi | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
3'-Biotin LC LC
3'-Biotin LC LC
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
3'-2LCBi | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
3'-Puromycin
3'-Puromycin
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
Pmn | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
3'-TAMRA
3'-TAMRA
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Extinction Coefficient:
Excitation/Emission Max: |
3'-TAM | $238.00 | $280.00 | $330.00 | $380.00 | $660.00 |
5'-Biotin
5'-Biotin
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
Bi | $90.00 | $95.00 | $105.00 | $155.00 | $270.00 |
5'-Cholesterol
5'-Cholesterol
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
Chl | $110.00 | $145.00 | $165.00 | $210.00 | $365.00 |
5' Cholesterol-TEG
5' Cholesterol-TEG
Modification Code:
Description:
Unit Structure: ![]()
Chemical Formula:
Unit Molecular Weight:
References:
|
Chl-TEG | $330.00 | $435.00 | $495.00 | $630.00 | $1,095.00 |
5'-Cy3
5'-Cy3
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Cy3 Extinction Coefficient:
Excitation/Emission Max: |
Cy3 | $75.00 | $95.00 | $115.00 | $135.00 | $250.00 |
5'-Cy5
5'-Cy5
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Cy5 Extinction Coefficient:
Excitation/Emission Max:
References: |
Cy5 | $75.00 | $95.00 | $115.00 | $135.00 | $250.00 |
5'-Cy5.5
5'-Cy5.5
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Cy5.5 Extinction Coefficient:
Excitation/Emission Max:
References: |
Cy5^5 | $75.00 | $95.00 | $115.00 | $135.00 | $250.00 |
5’ Black Hole Quencher 1
5’ Black Hole Quencher 1
|
BHQ-1 | - | $638.00 | $726.00 | $925.00 | $1609.00 |
5’ Black Hole Quencher 2
5’ Black Hole Quencher 2
|
BHQ-2 | - | $638.00 | $726.00 | $925.00 | $1609.00 |
5'-Dabcyl
5'-Dabcyl
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
References: |
Dabcyl | $100.00 | $130.00 | $145.00 | $180.00 | $320.00 |
5'-Fluorescein
5'-Fluorescein
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Extinction Coefficient:
Excitation/Emission Max: |
Fl | $90.00 | $95.00 | $105.00 | $135.00 | $230.00 |
5'-Pyrene
5'-Pyrene
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Pyrene Extinction Coefficient:
Excitation/Emission Max:
References: |
5'-Pyr | $115.00 | $155.00 | $190.00 | $240.00 | $460.00 |
5'-TAMRA
5'-TAMRA
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Extinction Coefficient:
Excitation/Emission Max: |
TAM | $238.00 | $280.00 | $330.00 | $380.00 | $660.00 |
5'-TET
5'-TET
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight:
Extinction Coefficient:
Excitation/Emission Max: |
TET | $75.00 | $93.00 | $105.00 | $130.00 | $230.00 |
Degenerate Bases | Short Code | 0.05 µmol | 0.2 µmol | 0.4 µmol | 1.0 µmol | 2.0 µmol |
Pricing shown is for first degenerate base coupling, subsequent additions at standard base pricing | ||||||
dN
dN
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
dN | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
dS
dS
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
dS | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
dW
dW
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
dW | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
mN
mN
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
mN | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
rN
rN
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
rN | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
rS
rS
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
rS | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
rW
rW
Modification Code:
Description: Unit Structure: ![]()
Unit Molecular Weight: |
rW | $45.00 | $52.00 | $63.00 | $78.00 | $135.00 |
Standard modifications for single-strand RNA can also be applied to siRNA by request. Please contact Technical Support to inquire about additional modifications or options for your siRNA, or request a quote now.
We routinely achieve 80-85% purity for unmodified siRNA strands without additional purification.
However, purification may be recommended when chemically synthesized siRNAs are:
- Chemically modified at the 3' end or internally
- Dually modified on the same strand
- Intended for use in highly sensitive assays or in vivo applications
Please contact Technical Support for information regarding recommendations for purification or purity estimates for unpurified material.
siRNA Processing Options:
Single Strands (A1) | Standard (A4) | HPLC | In vivo | In vivo HPLC | |
---|---|---|---|---|---|
Desalted | ✔ | ✔ | ✔ | ✔ | |
Deprotected | ✔ | ✔ | ✔ | ✔ | |
Duplexed | ✔ | ✔ | ✔ | ✔ | |
Purified | ✔ | ✔ | |||
Endotoxin tested | ✔ | ✔ | |||
Sodium counter-ion exchange | ✔ | ✔ | |||
Recommended for modified siRNA (dyes, etc.) | ✔ | ✔ | |||
Recommended for in vivo use | ✔ | ✔ | |||
2'ACE protected single-strands | ✔ |
Description of siRNA Processing Terms
- Desalted: The siRNA duplex has been desalted by either ethanol precipitation or C18 column desalting
- Deprotected: The 2'-ACE protecting groups of the RNA bases have been removed (deprotected)
- Duplexed: The two complementary siRNA strands have been annealed to form a duplex
- Single-strand (A1): The siRNA is provided with the sense and antisense strands in separate tubes; individual strands have NOT been desalted or deprotected
- Standard (A4): The siRNA is provided as a desalted & deprotected duplex, ready to use upon resuspension
- HPLC: The duplex has undergone ion exchange High Performance Liquid Chromatography for purification
- In vivo: The duplex has been processed by counter-ion (Na+) exchange, desalting, sterile filtration, and endotoxin testing
- In vivo HPLC: The duplex has undergone both in vivo processing as well as HPLC purification
Partner with the industry leader in RNAi technologies for the highest quality in vivo -ready RNAi reagents.
We want to ensure your in vivo experiments have the best chance of success. To assist you in your experimental planning, we offer the following options and guidance:
Assess your siRNA sequences in vitro
- We recommend identifying a highly functional siRNA design in vitro prior to initiating expensive experiments using animal models
- A predesigned Set of 4 siRNA reagents is ideal for testing multiple siRNA sequences
- Individual duplexes: Four individual siRNAs are pre-designed for every gene in human, mouse and rat. Order 1, 2, 3 or 4.
- SMARTpool: All four siRNAs are pooled together as a single reagent in one tube.
- Set of Four: All four siRNAs are provided as individual duplexes in four individual tubes.
Consider a proprietary siRNA modification pattern for enhanced nuclease resistance
- siSTABLE modifications prevent degradation from exo-and endonucleases. siSTABLE is recommended when the siRNA will be exposed to a biological environment rich in nucleases, such as animal serum.
- Accell modifications incorporate delivery-enhancing properties in addition to stability enhancement. Accell is recommended when target cell or tissue types are not amenable to standard modes of delivery.
-
See a list of recent publications using siSTABLE and Accell modified siRNA for in vivo experimentation.
- dye
ORDER custom siRNA with siSTABLE or Accell »
Carefully calculate the total siRNA quantity required
- Consider the number of animals in each cohort, the number of treatments or doses that will be required, and the amount of siRNA required for each dose
- In vivo experiments typically require a large quantity of siRNA. Synthesis capabilities can accommodate up to 10 grams by request, or up to 100 mg available online
- Need help converting nmol to mg? Check our siRNA yield table.
Consider in vivo processing of your siRNA when toxicity to the animal is of concern
- A specialized post-synthesis siRNA processing procedure including counter-ion (Na+) exchange, sterile filtration, desalting, and endotoxin testing
- Available with or without HPLC purification. For more information, review our siRNA purification and processing options.
- For more information on important considerations for in vivo experimentation see our Technical Note: " In vivo RNAi: Biodistribution, Delivery, and Applications"