Reliable gene silencing from the longest standing siRNA guarantee on the market.
The ON-TARGETplus 2.0, ON-TARGETplus, Accell and siGENOME siRNA lines are guaranteed* to effectively silence target gene expression by at least 75% at the mRNA level.
Knockdown long, non-coding RNA genes with specialized siRNA, modified to ensure specificity.
Use our siDESIGN Center to create a custom siRNA to any gene for any species or provide your own sequence for custom modified siRNA synthesis
A successful siRNA knockdown experiment must be both effective and specific. Horizon’s SMARTselection design algorithm (used for ON-TARGETplus 2.0, ON-TARGETplus, Accell, siGENOME and Lincode siRNA) provides a high degree of confidence that the targeted gene will be the only one silenced.
The primary source of siRNA-mediated off-targets is the seed region (nucleotides 2-7), which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. Incorporating comprehensive bioinformatic strategies such as seed region filters and seed frequency analysis within the SMARTselection design strategy minimizes microRNA-like off-target activity for improved specificity.
The SMARTpool (available for ON-TARGETplus 2.0, ON-TARGETplus, Accell, siGENOME and Lincode siRNA) combines four gene-specific siRNAs into a single reagent pool. Research has shown that pooled siRNAs:
Horizon offers multiple predesigned product lines across human, mouse and rat genomes. Use the table below to determine the optimal siRNA for your needs.
| Feature / Attribute | ON-TARGETplus 2.0 siRNA | ON-TARGETplus siRNA | Accell siRNA | siGENOME siRNA | Lincode siRNA |
|---|---|---|---|---|---|
| Key Benefit | Next-generation specificity with minimized off-target effects and improved consistency | High specificity for reduced off-targets + efficient silencing | Target silencing in difficult-to-transfect cells | Cost-effective, efficient silencing | Highly specific knockdown of long noncoding RNA (lncRNA) |
| Gene Coverage | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human, Mouse, and Rat protein-coding genes | Predesigned for Human and Mouse lncRNA |
| Best Use Case | Recommended for high-specificity screening, functional genomics, and follow-up validation | Recommended for transfectable mammalian cells | Recommended for neuronal, suspension, primary, and other hard-to-transfect cells | Recommended for transfectable mammalian cells | Highly specific lncRNA targeting |
| Transfection Requirements | DharmaFECT reagent recommended | DharmaFECT reagent recommended | None required | DharmaFECT reagent recommended | DharmaFECT reagent recommended |
| Available as SMARTpool | |||||
| Available as 4 individual siRNAs | |||||
| Guaranteed Knockdown (SMARTpool + 3 of 4) | (updated design enhances reliability) |
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| Sense Strand Modifications | Selective application as needed | ||||
| Antisense Seed Region Modification | |||||
| Uptake-Enhancing Modifications | Modifications to support delivery | ||||
| Nuclease Resistance | Stabilizing modifications included | ||||
| Sequence Information Provided | |||||
| Product Search | Search ON-TARGETplus 2.0 | Search ON-TARGETplus | Search Accell | Search siGENOME | Search Lincode |
Run your own siRNA knockdown screen at any scale with our predesigned and custom library options.
Find predesigned ON-TARGETplus and Accell libraries for druggable, gene family and whole genome screening.
Find predesigned ON-TARGETplus and siGENOME libraries for druggable, gene family and whole genome screening.
Design your own siRNA screening library and only screen the genes you need.
Need help with a new RNAi technique? We offer live scientific support as well as an extensive collection of technical resources to help get you started!
Choose the optimal siRNA product line and format for your experiment