cDNA and ORF clones provide a quick and accessible method for obtaining gene content for many in vitro and cell culture overexpression applications. The coding sequence is cloned into an expression vector under control of a strong promoter and then delivered to cells of interest by plasmid transfection or lentiviral transduction.

cDNA are ideal for overexpressing a gene in the context of native regulation, while ORFs have both 5' and 3' UTRs removed providing a shortcut to protein expression. They are most commonly used for gene overexpression studies, RNAi rescue experiments, structural analysis, visualization of protein localization, biochemical purification, and identification of interacting proteins through co-immunoprecipitation. See our gene overexpression applications page to learn more.


Benefits of using cDNA & ORF clones

Save time Have confidence Unlimited usage
Pre-cloned reagents eliminate time spent cloning. Expression ready and easy-to-transfer options allow you to focus on phenotypic results.
Human cDNA & ORF collections are sourced from trusted research groups with
fully sequenced options.
Overexpression clones are a "reusable resource" supporting unlimited experiments.


cDNA & ORF collections

cDNA & ORF collections are available as individual clones or in a variety of library formats

  • Mammalian Gene Collection
    Widely recognized as the gold standard for full-length, the MGC offers fully sequenced cDNA collections for human, mouse, rat and bovine genes.
  • Precision LentiORFs
    Human ORFs cloned into a lentiviral expression vector with a GFP reporter.
  • CCSB-Broad lentiviral expression library
    Human ORFS in lentiviral expression vector with a C-terminal V5 tag.
  • ORFeome Collaboration
    Sequence-verified, human open reading frames (ORFs) subcloned into Gateway® recombinational entry vectors.
  • CCSB hORFeome v5.1
    Gateway® adapted human entry ORFs are ideal for easily moving entire families of ORFs into your choice of protein expression systems.
  • CCSB hORFeome v8.1
    The latest version of the CCSB Human ORFeome library from Dana-Farber Cancer Institute.
  • Non-mammalian cDNA & ORFs
    Find cDNA and ORF clones for Yeast, E. coli, C. elegans, D. rerio, and Xenopus.

See our cDNA & ORF selection guide to learn more about each collection

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